Selective identification of secreted and transmembrane breast cancer markers using Escherichia coli ampicillin secretion trap

Deborah A. Ferguson, Matthew R. Muenster, Qun Zang, Jeffrey A. Spencer, Jeoffrey J. Schageman, Yun Lian, Harold R. Garner, Richard B. Gaynor, J. Warren Huff, Alexander Pertsemlidis, Raheela Ashfaq, John Schorge, Carlos Becerra, Noelle S. Williams, Jonathan M. Graff

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Secreted and cell surface proteins play important roles in cancer and are potential drug targets and tumor markers. Here, we describe a large-scale analysis of the genes encoding secreted and cell surface proteins in breast cancer. To identify these genes, we developed a novel signal sequence trap method called Escherichia coli ampicillin secretion trap (CAST). For CAST, we constructed a plasmid in which the signal sequence of β-lactamase was deleted such that it does not confer ampicillin resistance. Eukaryotic cDNA libraries cloned into pCAST produced tens of thousands of ampicillin-resistant clones, 80% of which contained cDNA fragments encoding secreted and membrane spanning proteins. We identified 2,708 unique sequences from cDNA libraries made from surgical breast cancer specimens. We analyzed the expression of 1,287 of the 2,708 genes and found that 166 were overexpressed in breast cancers relative to normal breast tissues. Eighty-five percent of these genes had not been previously identified as markers of breast cancer. Twenty-three of the 166 genes (14%) were relatively tissue restricted, suggesting use as cancer-specific targets. We also identified several new markers of ovarian cancer. Our results indicate that CAST is a robust, rapid, and low cost method to identify cell surface and secreted proteins and is applicable to a variety of relevant biological questions.

Original languageEnglish (US)
Pages (from-to)8209-8217
Number of pages9
JournalCancer Research
Volume65
Issue number18
DOIs
StatePublished - Sep 15 2005

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Ampicillin
Breast Neoplasms
Escherichia coli
Membrane Proteins
Genes
Protein Sorting Signals
Gene Library
Ampicillin Resistance
Tumor Biomarkers
Ovarian Neoplasms
Neoplasms
Breast
Plasmids
Complementary DNA
Clone Cells
Costs and Cost Analysis
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Selective identification of secreted and transmembrane breast cancer markers using Escherichia coli ampicillin secretion trap. / Ferguson, Deborah A.; Muenster, Matthew R.; Zang, Qun; Spencer, Jeffrey A.; Schageman, Jeoffrey J.; Lian, Yun; Garner, Harold R.; Gaynor, Richard B.; Huff, J. Warren; Pertsemlidis, Alexander; Ashfaq, Raheela; Schorge, John; Becerra, Carlos; Williams, Noelle S.; Graff, Jonathan M.

In: Cancer Research, Vol. 65, No. 18, 15.09.2005, p. 8209-8217.

Research output: Contribution to journalArticle

Ferguson, DA, Muenster, MR, Zang, Q, Spencer, JA, Schageman, JJ, Lian, Y, Garner, HR, Gaynor, RB, Huff, JW, Pertsemlidis, A, Ashfaq, R, Schorge, J, Becerra, C, Williams, NS & Graff, JM 2005, 'Selective identification of secreted and transmembrane breast cancer markers using Escherichia coli ampicillin secretion trap', Cancer Research, vol. 65, no. 18, pp. 8209-8217. https://doi.org/10.1158/0008-5472.CAN-04-3726
Ferguson, Deborah A. ; Muenster, Matthew R. ; Zang, Qun ; Spencer, Jeffrey A. ; Schageman, Jeoffrey J. ; Lian, Yun ; Garner, Harold R. ; Gaynor, Richard B. ; Huff, J. Warren ; Pertsemlidis, Alexander ; Ashfaq, Raheela ; Schorge, John ; Becerra, Carlos ; Williams, Noelle S. ; Graff, Jonathan M. / Selective identification of secreted and transmembrane breast cancer markers using Escherichia coli ampicillin secretion trap. In: Cancer Research. 2005 ; Vol. 65, No. 18. pp. 8209-8217.
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