Selective molecular assembly patterning: A new approach to micro- and nanochemical patterning of surfaces for biological applications

Roger Michel, Jost W. Lussi, Gabor Csucs, Ilya Reviakine, Gaudenz Danuser, Brigitte Ketterer, Jeffrey A. Hubbell, Marcus Textor, Nicholas D. Spencer

Research output: Contribution to journalArticle

156 Scopus citations

Abstract

A novel patterning technique based on selective self-assembly of alkane phosphates on metal oxide surfaces is presented. Standard photolithography was used to create patterns of titanium dioxide within a matrix of silicon dioxide. Alkane phosphates were found to self-assemble on TiO2, but not on SiO2, surfaces. Subsequent adsorption of poly(L-lysine)-g-poly(ethylene glycol) (PLL-g-PEG) rendered the exposed SiO2 surface resistant to protein adsorption. X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry were employed to monitor the assembly processes. Protein-adsorption studies by means of fluorescence microscopy conclusively established that the resulting surfaces displayed protein-adhesive, alkyl phosphate modified TiO2 features, arranged within a protein-resistant PLL-g-PEG-modified SiO2 matrix. Human foreskin fibroblasts, incubated in a serum-containing medium, were found to selectively attach to the protein-adhesive areas, where they developed focal contacts. No interaction of cells with the PLL-g-PEG-coated SiO2 areas was evident for at least 14 days. This patterning approach, termed selective molecular assembly patterning, is considered to be suitable for reproducible and cost-effective fabrication of biologically relevant chemical patterns over large areas.

Original languageEnglish (US)
Pages (from-to)3281-3287
Number of pages7
JournalLangmuir
Volume18
Issue number8
DOIs
StatePublished - Apr 16 2002

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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    Michel, R., Lussi, J. W., Csucs, G., Reviakine, I., Danuser, G., Ketterer, B., Hubbell, J. A., Textor, M., & Spencer, N. D. (2002). Selective molecular assembly patterning: A new approach to micro- and nanochemical patterning of surfaces for biological applications. Langmuir, 18(8), 3281-3287. https://doi.org/10.1021/la011715y