Selective role for DNMT3a in learning and memory

Michael J. Morris, Megumi Adachi, Elisa S. Na, Lisa M Monteggia

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Methylation of cytosine nucleotides is governed by DNA methyltransferases (DNMTs) that establish de novo DNA methylation patterns in early embryonic development (e.g., DNMT3a and DNMT3b) or maintain those patterns on hemimethylated DNA in dividing cells (e.g., DNMT1). DNMTs continue to be expressed at high levels in mature neurons, however their impact on neuronal function and behavior are unclear. To address this issue we examined DNMT1 and DNMT3a expression following associative learning. We also generated forebrain specific conditional Dnmt1 or Dnmt3a knockout mice and characterized them in learning and memory paradigms as well as for alterations in long-term potentiation (LTP) and synaptic plasticity. Here, we report that experience in an associative learning task impacts expression of Dnmt3a, but not Dnmt1, in brain areas that mediate learning of this task. We also found that Dnmt3a knockout mice, and not Dnmt1 knockouts have synaptic alterations as well as learning deficits on several associative and episodic memory tasks. These findings indicate that the de novo DNA methylating enzyme DNMT3a in postmitotic neurons is necessary for normal memory formation and its function cannot be substituted by the maintenance DNA methylating enzyme DNMT1.

Original languageEnglish (US)
Pages (from-to)30-37
Number of pages8
JournalNeurobiology of Learning and Memory
Volume115
DOIs
StatePublished - Nov 1 2014

Fingerprint

Learning
DNA
Methyltransferases
Knockout Mice
Cytosine Nucleotides
Neurons
Neuronal Plasticity
Episodic Memory
Long-Term Potentiation
DNA Methylation
Enzymes
Prosencephalon
Methylation
Embryonic Development
Maintenance
Brain

Keywords

  • Behavior
  • Excitatory neurotransmission
  • Fear conditioning
  • Long term potentiation
  • Methylation

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Cognitive Neuroscience
  • Experimental and Cognitive Psychology

Cite this

Selective role for DNMT3a in learning and memory. / Morris, Michael J.; Adachi, Megumi; Na, Elisa S.; Monteggia, Lisa M.

In: Neurobiology of Learning and Memory, Vol. 115, 01.11.2014, p. 30-37.

Research output: Contribution to journalArticle

Morris, Michael J. ; Adachi, Megumi ; Na, Elisa S. ; Monteggia, Lisa M. / Selective role for DNMT3a in learning and memory. In: Neurobiology of Learning and Memory. 2014 ; Vol. 115. pp. 30-37.
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