Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors.

Tae You Kwon, Shan Li Long, Nam Gyun Kim, Ju Kang Hyun, Hye Koh Kwi, Yong Joon Chwae, Mi Kim Kyoung, Ki Kim Yoon, Mi Park Sung, Key Jang Sung, Hoguen Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Frameshift and nonsense mutations are common in tumors with microsatellite instability, and mRNAs from these mutated genes have premature termination codons (PTCs). Abnormal mRNAs containing PTCs are normally degraded by the nonsense-mediated mRNA decay (NMD) system. However, PTCs located within 50-55 nucleotides of the last exon-exon junction are not recognized by NMD (NMD-irrelevant), and some PTC-containing mRNAs can escape from the NMD system (NMD-escape). We investigated protein expression from NMD-irrelevant and NMD-escape PTC-containing mRNAs by Western blotting and transfection assays. We demonstrated that transfection of NMD-irrelevant PTC-containing genomic DNA of MARCKS generates truncated protein. In contrast, NMD-escape PTC-containing versions of hMSH3 and TGFBR2 generate normal levels of mRNA, but do not generate detectable levels of protein. Transfection of NMD-escape mutant TGFBR2 genomic DNA failed to generate expression of truncated proteins, whereas transfection of wild-type TGFBR2 genomic DNA or mutant PTC-containing TGFBR2 cDNA generated expression of wild-type protein and truncated protein, respectively. Our findings suggest a novel mechanism of gene expression regulation for PTC-containing mRNAs in which the deleterious transcripts are regulated either by NMD or translational repression.

Original languageEnglish (US)
JournalPLoS Biology
Volume5
Issue number5
DOIs
StatePublished - Apr 2007

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Nonsense Mediated mRNA Decay
frameshift mutation
Frameshift Mutation
Nonsense Codon
stop codon
Tumors
deterioration
Messenger RNA
neoplasms
Neoplasms
Proteins
proteins
transfection
Transfection
genomics
exons
Exons
DNA
protein synthesis
nonsense mutation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors. / Kwon, Tae You; Long, Shan Li; Kim, Nam Gyun; Hyun, Ju Kang; Kwi, Hye Koh; Chwae, Yong Joon; Kyoung, Mi Kim; Yoon, Ki Kim; Sung, Mi Park; Sung, Key Jang; Kim, Hoguen.

In: PLoS Biology, Vol. 5, No. 5, 04.2007.

Research output: Contribution to journalArticle

Kwon, TY, Long, SL, Kim, NG, Hyun, JK, Kwi, HK, Chwae, YJ, Kyoung, MK, Yoon, KK, Sung, MP, Sung, KJ & Kim, H 2007, 'Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors.', PLoS Biology, vol. 5, no. 5. https://doi.org/10.1371/journal.pbio.0050109
Kwon, Tae You ; Long, Shan Li ; Kim, Nam Gyun ; Hyun, Ju Kang ; Kwi, Hye Koh ; Chwae, Yong Joon ; Kyoung, Mi Kim ; Yoon, Ki Kim ; Sung, Mi Park ; Sung, Key Jang ; Kim, Hoguen. / Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors. In: PLoS Biology. 2007 ; Vol. 5, No. 5.
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abstract = "Frameshift and nonsense mutations are common in tumors with microsatellite instability, and mRNAs from these mutated genes have premature termination codons (PTCs). Abnormal mRNAs containing PTCs are normally degraded by the nonsense-mediated mRNA decay (NMD) system. However, PTCs located within 50-55 nucleotides of the last exon-exon junction are not recognized by NMD (NMD-irrelevant), and some PTC-containing mRNAs can escape from the NMD system (NMD-escape). We investigated protein expression from NMD-irrelevant and NMD-escape PTC-containing mRNAs by Western blotting and transfection assays. We demonstrated that transfection of NMD-irrelevant PTC-containing genomic DNA of MARCKS generates truncated protein. In contrast, NMD-escape PTC-containing versions of hMSH3 and TGFBR2 generate normal levels of mRNA, but do not generate detectable levels of protein. Transfection of NMD-escape mutant TGFBR2 genomic DNA failed to generate expression of truncated proteins, whereas transfection of wild-type TGFBR2 genomic DNA or mutant PTC-containing TGFBR2 cDNA generated expression of wild-type protein and truncated protein, respectively. Our findings suggest a novel mechanism of gene expression regulation for PTC-containing mRNAs in which the deleterious transcripts are regulated either by NMD or translational repression.",
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