Self-assembly of heptameric nanoparticles derived from tag-functionalized Phi29 connectors

Dominik J. Green, Joseph C. Wang, Feng Xiao, Ying Cai, Rod Balhorn, Peixuan Guo, R. Holland Cheng

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The structure of an induced macromolecular assembly was characterized and found to consist of an ordered heptameric arrangement of recombinant phi29 gp10 connector molecules. Insertion of an N-terminal Strep-II/His6 tag to the connectors led to the spontaneous formation of large nanoparticles that were distinct from free, wild-type phi29 connectors in both size and symmetry elements. The determination of single-molecule tomograms and image-averaged reconstructions allowed for the stoichiometric and topological characterization of the ordered assemblage, revealing that the nanoparticle is composed of five equatorial connectors arranged with pseudo-5-fold rotational symmetry, capped on its ends by two polar connectors. Additionally, all seven connectors are oriented with their narrower N-terminal necks into the nanoparticle core and wider C-terminal ends out toward the nanoparticle surface, a geometric arrangement accommodated by the shape complementarity of the conical connector profiles. A significant amount of conformational heterogeneity was detected, ranging from changes in overall nanoparticle diameter, to tilting of individual connectors, to variations in connector stoichiometry. Nevertheless, a stable, heptameric nanoparticle was resolved, revealing the significant potential of guided, peptide-mediated supramolecular self-assembly. With this construct, we anticipate the further design of variable N-terminal tags to allow for the generation of nanoparticles with tailored connector stoichiometry and topological arrangements. By modifying the surface-exposed C-terminal ends with application-appropriate moieties, the consistent structure and compact nature of these nanoparticles may prove beneficial in nanotechnological and nanomedical approaches.

Original languageEnglish (US)
Pages (from-to)7651-7659
Number of pages9
JournalACS Nano
Volume4
Issue number12
DOIs
StatePublished - Dec 28 2010
Externally publishedYes

Fingerprint

connectors
Self assembly
self assembly
Nanoparticles
nanoparticles
His-His-His-His-His-His
Stoichiometry
stoichiometry
Molecules
Image reconstruction
Peptides
symmetry
image reconstruction
peptides
molecules
insertion
assembly

Keywords

  • conformational heterogeneity
  • DNA motor
  • gp10 connector
  • MSA
  • peptide tag
  • phi29
  • self-assembly

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Green, D. J., Wang, J. C., Xiao, F., Cai, Y., Balhorn, R., Guo, P., & Cheng, R. H. (2010). Self-assembly of heptameric nanoparticles derived from tag-functionalized Phi29 connectors. ACS Nano, 4(12), 7651-7659. https://doi.org/10.1021/nn1024829

Self-assembly of heptameric nanoparticles derived from tag-functionalized Phi29 connectors. / Green, Dominik J.; Wang, Joseph C.; Xiao, Feng; Cai, Ying; Balhorn, Rod; Guo, Peixuan; Cheng, R. Holland.

In: ACS Nano, Vol. 4, No. 12, 28.12.2010, p. 7651-7659.

Research output: Contribution to journalArticle

Green, DJ, Wang, JC, Xiao, F, Cai, Y, Balhorn, R, Guo, P & Cheng, RH 2010, 'Self-assembly of heptameric nanoparticles derived from tag-functionalized Phi29 connectors', ACS Nano, vol. 4, no. 12, pp. 7651-7659. https://doi.org/10.1021/nn1024829
Green, Dominik J. ; Wang, Joseph C. ; Xiao, Feng ; Cai, Ying ; Balhorn, Rod ; Guo, Peixuan ; Cheng, R. Holland. / Self-assembly of heptameric nanoparticles derived from tag-functionalized Phi29 connectors. In: ACS Nano. 2010 ; Vol. 4, No. 12. pp. 7651-7659.
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