Self-calibrating viscosity probes: Design and subcellular localization

Marianna Dakanali, Thai H. Do, Austin Horn, Akaraphon Chongchivivat, Tuptim Jarusreni, Darcy Lichlyter, Gianni Guizzunti, Mark A. Haidekker, Emmanuel A. Theodorakis

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


We describe the design, synthesis and fluorescence profiles of new self-calibrating viscosity dyes in which a coumarin (reference fluorophore) has been covalently linked with a molecular rotor (viscosity sensor). Characterization of their fluorescence properties was made with separate excitation of the units and through resonance energy transfer from the reference to the sensor dye. We have modified the linker and the substitution of the rotor in order to change the hydrophilicity of these probes thereby altering their subcellular localization. For instance, hydrophilic dye 12 shows a homogeneous distribution inside the cell and represents a suitable probe for viscosity measurements in the cytoplasm.

Original languageEnglish (US)
Pages (from-to)4443-4450
Number of pages8
JournalBioorganic and Medicinal Chemistry
Issue number14
StatePublished - Jul 15 2012


  • Fluorescence
  • Molecular rotor
  • Ratiometric dye
  • Subcellular viscosity

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry


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