Self-calibrating viscosity probes: Design and subcellular localization

Marianna Dakanali, Thai H. Do, Austin Horn, Akaraphon Chongchivivat, Tuptim Jarusreni, Darcy Lichlyter, Gianni Guizzunti, Mark A. Haidekker, Emmanuel A. Theodorakis

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We describe the design, synthesis and fluorescence profiles of new self-calibrating viscosity dyes in which a coumarin (reference fluorophore) has been covalently linked with a molecular rotor (viscosity sensor). Characterization of their fluorescence properties was made with separate excitation of the units and through resonance energy transfer from the reference to the sensor dye. We have modified the linker and the substitution of the rotor in order to change the hydrophilicity of these probes thereby altering their subcellular localization. For instance, hydrophilic dye 12 shows a homogeneous distribution inside the cell and represents a suitable probe for viscosity measurements in the cytoplasm.

Original languageEnglish (US)
Pages (from-to)4443-4450
Number of pages8
JournalBioorganic and Medicinal Chemistry
Volume20
Issue number14
DOIs
StatePublished - Jul 15 2012

Keywords

  • Fluorescence
  • Molecular rotor
  • Ratiometric dye
  • Subcellular viscosity

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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  • Cite this

    Dakanali, M., Do, T. H., Horn, A., Chongchivivat, A., Jarusreni, T., Lichlyter, D., Guizzunti, G., Haidekker, M. A., & Theodorakis, E. A. (2012). Self-calibrating viscosity probes: Design and subcellular localization. Bioorganic and Medicinal Chemistry, 20(14), 4443-4450. https://doi.org/10.1016/j.bmc.2012.05.026