Senescing human bone-marrow-derived clonal mesenchymal stem cells have altered lysophospholipid composition and functionality

Seul Ji Lee, Tacghee Yi, Soo Hyun Ahn, Dong Kyu Lim, Ji Yeon Hong, Yun Kyoung Cho, Johan Lim, Sun U. Song, Sung Won Kwon

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Mesenchymal stem cells (MSCs) have been used in a wide range of research and clinical studies because MSCs do not have any ethical issues and have the advantage of low carcinogenicity due to their limited proliferation. However, because only a small number of MSCs can be obtained from the bone marrow, ex vivo amplification is inevitably required. For that reason, this study was conducted to acquire the metabolic information to examine and control the changes in the activities and differentiation potency of MSCs during the ex vivo culture process. Endogenous metabolites of human bone-marrow-derived clonal MSCs (hcMSCs) during cellular senescence were profiled by ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/QTOFMS). To select significant metabolites, we used the linear mixed effects model having fixed effects for batch and time (passage) and random effects for metabolites, determining the mean using a t test and the standard deviation using an F test. We used structural analysis with representative standards and spectrum patterns with different collision energies to distinctly identify eight metabolites with altered expression during senescence as types of lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), such as LPC 16:0 and LPE 22:4. The present study revealed changes in endogenous metabolites and mechanisms due to senescence.

Original languageEnglish (US)
Pages (from-to)1438-1449
Number of pages12
JournalJournal of Proteome Research
Volume13
Issue number3
DOIs
StatePublished - Mar 7 2014

Keywords

  • cellular senescence
  • human clonal mesenchymal stem cells
  • lysophosphatidylcholine
  • lysophosphatidylethanolamine

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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    Lee, S. J., Yi, T., Ahn, S. H., Lim, D. K., Hong, J. Y., Cho, Y. K., Lim, J., Song, S. U., & Kwon, S. W. (2014). Senescing human bone-marrow-derived clonal mesenchymal stem cells have altered lysophospholipid composition and functionality. Journal of Proteome Research, 13(3), 1438-1449. https://doi.org/10.1021/pr400990k