We have investigated the sensitivity to DNA-damaging agents of a strain of Saccharomyces cerevisiae containing a deletion of the RAD27 gene. The mutant strain is sensitive to a number of alkylating agents that modify DNA at a variety of positions, including one that produces primarily phosphotriesters. In contrast, the mutant strain is not sensitive to the oxidizing agent hydrogen peroxide. The introduction of a plasmid containing the FEN-1 gene (the human ortholog of the RAD27 gene) can substantially complement the sensitivity to alkylating agents observed in the mutant strain. (C) 2000 Elsevier Science B.V.
|Original language||English (US)|
|Number of pages||6|
|Journal||Mutation Research - DNA Repair|
|State||Published - Nov 9 2000|
- Base excision repair
ASJC Scopus subject areas
- Molecular Biology