Abstract
We have investigated the sensitivity to DNA-damaging agents of a strain of Saccharomyces cerevisiae containing a deletion of the RAD27 gene. The mutant strain is sensitive to a number of alkylating agents that modify DNA at a variety of positions, including one that produces primarily phosphotriesters. In contrast, the mutant strain is not sensitive to the oxidizing agent hydrogen peroxide. The introduction of a plasmid containing the FEN-1 gene (the human ortholog of the RAD27 gene) can substantially complement the sensitivity to alkylating agents observed in the mutant strain. (C) 2000 Elsevier Science B.V.
Original language | English (US) |
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Pages (from-to) | 243-248 |
Number of pages | 6 |
Journal | Mutation Research - DNA Repair |
Volume | 461 |
Issue number | 3 |
DOIs | |
State | Published - Nov 9 2000 |
Keywords
- Alkylation
- Base excision repair
- FEN-1
- Rad27
ASJC Scopus subject areas
- Molecular Biology
- Toxicology
- Genetics