Sensitivity of a S. cerevisiae RAD27 deletion mutant to DNA-damaging agents and in vivo complementation by the human FEN-1 gene

Ryan J. Hansen, Errol C. Friedberg, Michael S. Reagan

Research output: Contribution to journalArticle

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Abstract

We have investigated the sensitivity to DNA-damaging agents of a strain of Saccharomyces cerevisiae containing a deletion of the RAD27 gene. The mutant strain is sensitive to a number of alkylating agents that modify DNA at a variety of positions, including one that produces primarily phosphotriesters. In contrast, the mutant strain is not sensitive to the oxidizing agent hydrogen peroxide. The introduction of a plasmid containing the FEN-1 gene (the human ortholog of the RAD27 gene) can substantially complement the sensitivity to alkylating agents observed in the mutant strain. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)243-248
Number of pages6
JournalMutation Research - DNA Repair
Volume461
Issue number3
DOIs
StatePublished - Nov 9 2000

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Keywords

  • Alkylation
  • Base excision repair
  • FEN-1
  • Rad27

ASJC Scopus subject areas

  • Molecular Biology
  • Toxicology
  • Genetics

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