Sequence-dependent conformational variations in the b-dna double-helix of poly d(Aatt) poly d(aatt)

R. Chandrasekaran, A. Radha, R. L. Ratliff

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

X-ray diffraction data from well oriented and polycrystalline fibers of the lithium salt of poly d(AATT) poly d(AATT) are isomorphous with those from B-DNA The double-helix consists of conformationally identical antiparallel strands and the molecular symmetry is 2 52; the asymmetric unit is a tetranucleotide, AATT, and 5 tetranucleotides span two turns per strand. Two double helices pass through a monoclinic unit cell of dimensions a = 31.05, b = 22.62, c = 33.85 Å (fiber axis) and γ = 90°. In each repeating motif, the four nucleotides have distinct conformations, TpA displays an axial P-O bond and there is shortening of minor groove in the central region.

Original languageEnglish (US)
Pages (from-to)741-766
Number of pages26
JournalJournal of Biomolecular Structure and Dynamics
Volume11
Issue number4
DOIs
StatePublished - Feb 1994

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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