Sequence-dependent conformational variations in the B-DNA double-helix of poly d(AATT)·poly d(AATT)

R. Chandrasekaran, A. Radha, R. L. Ratliff

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

X-ray diffraction data from well oriented and polycrystalline fibers of the lithium salt of poly d(AATT)·poly d(AATT) are isomorphous with those from B-DNA. The double-helix consists of conformationally identical antiparallel strands and the molecular symmetry is 2 52; the asymmetric unit is a tetranucleotide, AATT, and 5 tetranucleotides span two turns per strand. Two double helices pass through a monoclinic unit cell of dimensions a = 31.05, b = 22.62, c = 33.85 Å (fiber axis) and γ = 90°. In each repeating motif, the four nucleotides have distinct conformations, TpA displays an axial P-O bond and there is shortening of minor groove in the central region.

Original languageEnglish (US)
Pages (from-to)741-766
Number of pages26
JournalJournal of Biomolecular Structure and Dynamics
Volume11
Issue number4
StatePublished - 1994

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B-Form DNA
Lithium
X-Ray Diffraction
Nucleotides
Salts

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Sequence-dependent conformational variations in the B-DNA double-helix of poly d(AATT)·poly d(AATT). / Chandrasekaran, R.; Radha, A.; Ratliff, R. L.

In: Journal of Biomolecular Structure and Dynamics, Vol. 11, No. 4, 1994, p. 741-766.

Research output: Contribution to journalArticle

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