Sequence specific detection of bacterial 23S ribosomal RNA by TLR13

Xiao Dong Li, Zhijian J. Chen

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Toll-like receptors (TLRs) detect microbial infections and trigger innate immune responses. Among vertebrate TLRs, the role of TLR13 and its ligand are unknown. Here we show that TLR13 detects the 23S ribosomal RNA of both gram-positive and gram-negative bacteria. A sequence containing 13 nucleotides near the active site of 23S rRNA ribozyme, which catalyzes peptide bond synthesis, was both necessary and sufficient to trigger TLR13-dependent interleukin-1β production. Single point mutations within this sequence destroyed the ability of the 23S rRNA to stimulate the TLR13 pathway. Knockout of TLR13 in mice abolished the induction of interleukin-1β and other cytokines by the 23S rRNA sequence. Thus, TLR13 detects bacterial RNA with exquisite sequence specificity.

Original languageEnglish (US)
Article numbere00102
JournaleLife
Volume2012
Issue number1
DOIs
StatePublished - Oct 30 2012

Fingerprint

23S Ribosomal RNA
Toll-Like Receptors
Interleukin-1
Bacterial RNA
Catalytic RNA
Gram-Negative Bacteria
Point Mutation
Innate Immunity
Vertebrates
Catalytic Domain
Bacteria
Nucleotides
Cytokines
Ligands
Peptides
Infection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Sequence specific detection of bacterial 23S ribosomal RNA by TLR13. / Li, Xiao Dong; Chen, Zhijian J.

In: eLife, Vol. 2012, No. 1, e00102, 30.10.2012.

Research output: Contribution to journalArticle

@article{df307dacff6f4c93a6b7cb8c36a43347,
title = "Sequence specific detection of bacterial 23S ribosomal RNA by TLR13",
abstract = "Toll-like receptors (TLRs) detect microbial infections and trigger innate immune responses. Among vertebrate TLRs, the role of TLR13 and its ligand are unknown. Here we show that TLR13 detects the 23S ribosomal RNA of both gram-positive and gram-negative bacteria. A sequence containing 13 nucleotides near the active site of 23S rRNA ribozyme, which catalyzes peptide bond synthesis, was both necessary and sufficient to trigger TLR13-dependent interleukin-1β production. Single point mutations within this sequence destroyed the ability of the 23S rRNA to stimulate the TLR13 pathway. Knockout of TLR13 in mice abolished the induction of interleukin-1β and other cytokines by the 23S rRNA sequence. Thus, TLR13 detects bacterial RNA with exquisite sequence specificity.",
author = "Li, {Xiao Dong} and Chen, {Zhijian J.}",
year = "2012",
month = "10",
day = "30",
doi = "10.7554/eLife.00102",
language = "English (US)",
volume = "2012",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
number = "1",

}

TY - JOUR

T1 - Sequence specific detection of bacterial 23S ribosomal RNA by TLR13

AU - Li, Xiao Dong

AU - Chen, Zhijian J.

PY - 2012/10/30

Y1 - 2012/10/30

N2 - Toll-like receptors (TLRs) detect microbial infections and trigger innate immune responses. Among vertebrate TLRs, the role of TLR13 and its ligand are unknown. Here we show that TLR13 detects the 23S ribosomal RNA of both gram-positive and gram-negative bacteria. A sequence containing 13 nucleotides near the active site of 23S rRNA ribozyme, which catalyzes peptide bond synthesis, was both necessary and sufficient to trigger TLR13-dependent interleukin-1β production. Single point mutations within this sequence destroyed the ability of the 23S rRNA to stimulate the TLR13 pathway. Knockout of TLR13 in mice abolished the induction of interleukin-1β and other cytokines by the 23S rRNA sequence. Thus, TLR13 detects bacterial RNA with exquisite sequence specificity.

AB - Toll-like receptors (TLRs) detect microbial infections and trigger innate immune responses. Among vertebrate TLRs, the role of TLR13 and its ligand are unknown. Here we show that TLR13 detects the 23S ribosomal RNA of both gram-positive and gram-negative bacteria. A sequence containing 13 nucleotides near the active site of 23S rRNA ribozyme, which catalyzes peptide bond synthesis, was both necessary and sufficient to trigger TLR13-dependent interleukin-1β production. Single point mutations within this sequence destroyed the ability of the 23S rRNA to stimulate the TLR13 pathway. Knockout of TLR13 in mice abolished the induction of interleukin-1β and other cytokines by the 23S rRNA sequence. Thus, TLR13 detects bacterial RNA with exquisite sequence specificity.

UR - http://www.scopus.com/inward/record.url?scp=84877579519&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877579519&partnerID=8YFLogxK

U2 - 10.7554/eLife.00102

DO - 10.7554/eLife.00102

M3 - Article

C2 - 23110254

AN - SCOPUS:84877579519

VL - 2012

JO - eLife

JF - eLife

SN - 2050-084X

IS - 1

M1 - e00102

ER -