TY - JOUR
T1 - Sequences involved in mRNA processing in Trypanosoma cruzi
AU - Campos, Priscila C.
AU - Bartholomeu, Daniella C.
AU - DaRocha, Wanderson D.
AU - Cerqueira, Gustavo C.
AU - Teixeira, Santuza M.R.
N1 - Funding Information:
This work was supported by grants from CNPq and FAPEMIG, Brazil, by UNICEF/UNDP/World Bank WHO for Research and Training in Tropical Diseases and by an International Research Scholars Grant from the Howard Hughes Medical Institute.
PY - 2008/10
Y1 - 2008/10
N2 - Gene expression in Trypanosomatids requires processing of polycistronic transcripts to generate monocistronic mRNAs by cleavage events that are coupled to the addition of a Spliced Leader sequence (SL) at the 5′-end and a poly(A) tail at the 3′-end of each mRNA. Here we investigate the sequence requirements involved in Trypanosoma cruzi mRNA processing by mapping all available expressed sequence tags and cDNAs containing poly(A) tail and/or SL to genomic intergenic regions. Amongst other parameters, we determined that the median lengths of 5′ untranslated region (UTR) and 3′UTR sequences are 35 and 264 nucleotides, respectively; and that the median distance between SL addition sites and a polypyrimidine motif is 18 nucleotides, whereas the median distance between poly(A) addition sites and the closest polypyrimidine-rich sequence is 40 nucleotides.
AB - Gene expression in Trypanosomatids requires processing of polycistronic transcripts to generate monocistronic mRNAs by cleavage events that are coupled to the addition of a Spliced Leader sequence (SL) at the 5′-end and a poly(A) tail at the 3′-end of each mRNA. Here we investigate the sequence requirements involved in Trypanosoma cruzi mRNA processing by mapping all available expressed sequence tags and cDNAs containing poly(A) tail and/or SL to genomic intergenic regions. Amongst other parameters, we determined that the median lengths of 5′ untranslated region (UTR) and 3′UTR sequences are 35 and 264 nucleotides, respectively; and that the median distance between SL addition sites and a polypyrimidine motif is 18 nucleotides, whereas the median distance between poly(A) addition sites and the closest polypyrimidine-rich sequence is 40 nucleotides.
KW - Polypyrimidine
KW - Trypanosoma cruzi
KW - Untranslated regions
KW - mRNA processing
KW - trans-splicing
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U2 - 10.1016/j.ijpara.2008.07.001
DO - 10.1016/j.ijpara.2008.07.001
M3 - Article
C2 - 18700146
AN - SCOPUS:51249103596
SN - 0020-7519
VL - 38
SP - 1383
EP - 1389
JO - International Journal for Parasitology
JF - International Journal for Parasitology
IS - 12
ER -