Sequences involved in mRNA processing in Trypanosoma cruzi

Priscila C. Campos, Daniella C. Bartholomeu, Wanderson D. DaRocha, Gustavo C. Cerqueira, Santuza M.R. Teixeira

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Gene expression in Trypanosomatids requires processing of polycistronic transcripts to generate monocistronic mRNAs by cleavage events that are coupled to the addition of a Spliced Leader sequence (SL) at the 5′-end and a poly(A) tail at the 3′-end of each mRNA. Here we investigate the sequence requirements involved in Trypanosoma cruzi mRNA processing by mapping all available expressed sequence tags and cDNAs containing poly(A) tail and/or SL to genomic intergenic regions. Amongst other parameters, we determined that the median lengths of 5′ untranslated region (UTR) and 3′UTR sequences are 35 and 264 nucleotides, respectively; and that the median distance between SL addition sites and a polypyrimidine motif is 18 nucleotides, whereas the median distance between poly(A) addition sites and the closest polypyrimidine-rich sequence is 40 nucleotides.

Original languageEnglish (US)
Pages (from-to)1383-1389
Number of pages7
JournalInternational Journal for Parasitology
Volume38
Issue number12
DOIs
StatePublished - Oct 2008
Externally publishedYes

Keywords

  • mRNA processing
  • Polypyrimidine
  • trans-splicing
  • Trypanosoma cruzi
  • Untranslated regions

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Sequences involved in mRNA processing in Trypanosoma cruzi'. Together they form a unique fingerprint.

Cite this