Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases

Makio Iwashima, Bryan A. Irving, Nicolai S C Van Oers, Andrew C. Chan, Arthur Weiss

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601 Citations (Scopus)

Abstract

The T cell antigen receptor (TCR) initiates signals by interacting with cytoplasmic protein tyrosine kinases (PTKs) through a 17-residue sequence motif [called the antigen recognition activation motif (ARAM)] that is contained in the TCRζ and CD3 chains. TCR stimulation induces the tyrosine phosphorylation of several cellular substrates, including the ARAMs. Lck kinase activity is required for phosphorylation of two conserved tyrosine residues in an ARAM. This phosphorylation leads to the recruitment of a second cytoplasmic PTK, ZAP-70, through both of the ZAP-70 Src homology 2 domains and its phosphorylation. Thus, TCR signal transduction is initiated by the sequential interaction of two PTKs with TCR ARAMs.

Original languageEnglish (US)
Pages (from-to)1136-1139
Number of pages4
JournalScience
Volume263
Issue number5150
StatePublished - Feb 25 1994

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T-Cell Antigen Receptor
Protein-Tyrosine Kinases
Phosphorylation
Tyrosine
ZAP-70 Protein-Tyrosine Kinase
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Antigens
src Homology Domains
Signal Transduction

ASJC Scopus subject areas

  • General

Cite this

Iwashima, M., Irving, B. A., Van Oers, N. S. C., Chan, A. C., & Weiss, A. (1994). Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases. Science, 263(5150), 1136-1139.

Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases. / Iwashima, Makio; Irving, Bryan A.; Van Oers, Nicolai S C; Chan, Andrew C.; Weiss, Arthur.

In: Science, Vol. 263, No. 5150, 25.02.1994, p. 1136-1139.

Research output: Contribution to journalArticle

Iwashima, M, Irving, BA, Van Oers, NSC, Chan, AC & Weiss, A 1994, 'Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases', Science, vol. 263, no. 5150, pp. 1136-1139.
Iwashima M, Irving BA, Van Oers NSC, Chan AC, Weiss A. Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases. Science. 1994 Feb 25;263(5150):1136-1139.
Iwashima, Makio ; Irving, Bryan A. ; Van Oers, Nicolai S C ; Chan, Andrew C. ; Weiss, Arthur. / Sequential interactions of the TCR with two distinct cytoplasmic tyrosine kinases. In: Science. 1994 ; Vol. 263, No. 5150. pp. 1136-1139.
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