Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115

James Shorter, Matthew B. Beard, Joachim Seemann, A. Barbara Dirac-Svejstrup, Graham Warren

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

p115 tethers coat protein (COP)I vesicles to Golgi membranes. The acidic COOH-terminal domain of p115 links the Golgins, Giantin on COPI vesicles, to GM130 on Golgi membranes. We now show that a SNARE motif-related domain within p115 stimulates the specific assembly of endogenous Golgi SNAREpins containing the t-SNARE, syntaxin 5. p115 catalyzes the construction of a cognate GOS-28-syntaxin-5 (v-/t-SNARE) complex by first linking the SNAREs to promote their direct interaction. These events are essential for NSF-catalyzed reassembly of postmitotic Golgi vesicles and tubules into mature cisternae. Staging experiments reveal that the linking of Golgins precedes SNAREpin assembly. Thus, p115 coordinates sequential tethering and docking of COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs).

Original languageEnglish (US)
Pages (from-to)45-62
Number of pages18
JournalJournal of Cell Biology
Volume157
Issue number1
DOIs
StatePublished - Apr 1 2002

Fingerprint

SNARE Proteins
Catalysis
Coat Protein Complex I
Qa-SNARE Proteins
Proteins
Membranes
Capsid Proteins

Keywords

  • Golgi apparatus
  • Golgin
  • Mitosis
  • P115
  • SNAREpin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115. / Shorter, James; Beard, Matthew B.; Seemann, Joachim; Barbara Dirac-Svejstrup, A.; Warren, Graham.

In: Journal of Cell Biology, Vol. 157, No. 1, 01.04.2002, p. 45-62.

Research output: Contribution to journalArticle

Shorter, James ; Beard, Matthew B. ; Seemann, Joachim ; Barbara Dirac-Svejstrup, A. ; Warren, Graham. / Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115. In: Journal of Cell Biology. 2002 ; Vol. 157, No. 1. pp. 45-62.
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