Serial determination of glomerular filtration rate in conscious mice using FITC-inulin clearance

Zhonghua Qi, Irene Whitt, Amit Mehta, Jianping Jin, Min Zhao, Raymond C. Harris, Agnes B. Fogo, Matthew D. Breyer

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229 Scopus citations

Abstract

Two nonradioactive methods for determining glomerular filtration rate (GFR) in conscious mice using FITC-labeled inulin (FITC-inulin) were evaluated. The first method measured GFR using clearance kinetics of plasma FITC-inulin after a single bolus injection. Based on a two-compartment model, estimated GFR was 236.69 ± 16.55 and 140.20 ± 22.27 μl/min in male and female C57BL/6J mice, respectively. Total or 5/6 nephrectomy reduced inulin clearance to 0 or 32.80 ± 9.32 μl/min, respectively. Conversely, diabetes mellitus induced by streptozotocin was associated with increased GFR. The other approach measured urinary inulin clearance using intraperitoneal microosmotic pumps to deliver FITC-inulin and metabolic cages to collect timed urine samples. This approach yielded similar GFR values of 211.11 ± 26.56 and 157.36 ± 20.02 μl/min in male and female mice, respectively. These studies demonstrate the feasibility of repeated nonisotopic measurement of inulin clearance in conscious mice.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume286
Issue number3 55-3
Publication statusPublished - Mar 2004

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Keywords

  • Diabetes mellitus
  • Hemodynamics
  • Nephrectomy
  • Salts

ASJC Scopus subject areas

  • Physiology

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