Serial determination of glomerular filtration rate in conscious mice using FITC-inulin clearance

Zhonghua Qi, Irene Whitt, Amit Mehta, Jianping Jin, Min Zhao, Raymond C. Harris, Agnes B. Fogo, Matthew D. Breyer

Research output: Contribution to journalArticle

223 Citations (Scopus)

Abstract

Two nonradioactive methods for determining glomerular filtration rate (GFR) in conscious mice using FITC-labeled inulin (FITC-inulin) were evaluated. The first method measured GFR using clearance kinetics of plasma FITC-inulin after a single bolus injection. Based on a two-compartment model, estimated GFR was 236.69 ± 16.55 and 140.20 ± 22.27 μl/min in male and female C57BL/6J mice, respectively. Total or 5/6 nephrectomy reduced inulin clearance to 0 or 32.80 ± 9.32 μl/min, respectively. Conversely, diabetes mellitus induced by streptozotocin was associated with increased GFR. The other approach measured urinary inulin clearance using intraperitoneal microosmotic pumps to deliver FITC-inulin and metabolic cages to collect timed urine samples. This approach yielded similar GFR values of 211.11 ± 26.56 and 157.36 ± 20.02 μl/min in male and female mice, respectively. These studies demonstrate the feasibility of repeated nonisotopic measurement of inulin clearance in conscious mice.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume286
Issue number3 55-3
StatePublished - Mar 2004

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Glomerular Filtration Rate
Inulin
Feasibility Studies
Streptozocin
Nephrectomy
Inbred C57BL Mouse
Diabetes Mellitus
FITC-inulin
Urine
Injections

Keywords

  • Diabetes mellitus
  • Hemodynamics
  • Nephrectomy
  • Salts

ASJC Scopus subject areas

  • Physiology

Cite this

Serial determination of glomerular filtration rate in conscious mice using FITC-inulin clearance. / Qi, Zhonghua; Whitt, Irene; Mehta, Amit; Jin, Jianping; Zhao, Min; Harris, Raymond C.; Fogo, Agnes B.; Breyer, Matthew D.

In: American Journal of Physiology - Renal Physiology, Vol. 286, No. 3 55-3, 03.2004.

Research output: Contribution to journalArticle

Qi, Zhonghua ; Whitt, Irene ; Mehta, Amit ; Jin, Jianping ; Zhao, Min ; Harris, Raymond C. ; Fogo, Agnes B. ; Breyer, Matthew D. / Serial determination of glomerular filtration rate in conscious mice using FITC-inulin clearance. In: American Journal of Physiology - Renal Physiology. 2004 ; Vol. 286, No. 3 55-3.
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