Serial Non-Invasive Assessment of Antibody Induced Nephritis in Mice Using Positron Emission Tomography

Guiyang Hao, Yong Du, Xin J. Zhou, Jianfei Guo, Xiankai Sun, Chandra Mohan, Orhan K. Öz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Mouse models of experimental anti-glomerular basement membrane (anti-GBM) nephritis provide an analytical tool for studying spontaneous lupus nephritis. The potential of Positron Emission Tomography (PET) was evaluated using 2-deoxy-2-[18F]fluoro-d-glucose (FDG) as a probe to monitor the progression of anti-GBM induced nephritis in a mouse model. The imaging results were compared to conventional measures of renal function and pathological changes. Serum and urinary vascular cell adhesion molecule-1 (VCAM-1) levels were used as measures of endothelial cell activation and inflammation. Following a challenge with anti-glomerular antibodies, mice exhibited peak changes in serum creatinine, proteinuria, and glomerulonephritis score at 14 days post-challenge (p.c.). In contrast, VCAM levels peaked at day 7 p.c. On dynamic PET images (0-60 min) of day 7, kidneys of the anti-GBM nephritis mice demonstrated a unique pattern of FDG uptake. Compared to the time activity curve (TAC) prior to challenge, a rightward shift was observed after the challenge. By day 10 p.c., kidney FDG uptake was lower than baseline and remained so until the study ended at 21 days p.c. During this time frame measures of renal dysfunction remained high but VCAM-1 levels declined. These changes were accompanied by an increase in kidney volume as measured by Computed Tomography (CT) and intra-abdominal fluid collection. Our results suggest that FDG-PET-CT can be used as a non-invasive imaging tool to longitudinally monitor the progression of renal disease activity in antibody mediated nephritis and the magnitude of renal FDG retention correlates better with early markers of renal inflammation than renal dysfunction.

Original languageEnglish (US)
Article numbere57418
JournalPLoS One
Volume8
Issue number2
DOIs
StatePublished - Feb 27 2013

Fingerprint

positron-emission tomography
nephritis
Positron emission tomography
Nephritis
Positron-Emission Tomography
Vascular Cell Adhesion Molecule-1
kidneys
Kidney
antibodies
Tomography
Antibodies
mice
basement membrane
Imaging techniques
Glomerular Basement Membrane
Endothelial cells
computed tomography
Creatinine
cell adhesion
blood vessels

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Serial Non-Invasive Assessment of Antibody Induced Nephritis in Mice Using Positron Emission Tomography. / Hao, Guiyang; Du, Yong; Zhou, Xin J.; Guo, Jianfei; Sun, Xiankai; Mohan, Chandra; Öz, Orhan K.

In: PLoS One, Vol. 8, No. 2, e57418, 27.02.2013.

Research output: Contribution to journalArticle

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