Serial phenotypic, cytogenetic and molecular genetic studies in Richter's syndrome: Demonstration of lymphoma development from the chronic lymphocytic leukaemia cells

P. R K Koduru, S. M. Lichtman, T. F. Smilari, T. Sun, J. C. Goh, L. Karp, W. Hall, S. Hashimoto, N. Chiorazzi, J. D. Broome

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In this report we describe a unique longitudinal study on the clinical, phenotypic, cytogenetic and molecular genetic features of malignant cells from diagnosis of chronic lymphocytic leukaemia (CLL) to the development of lymphoma and lymphomatous meningitis. CLL cells at diagnosis were CD5+, CD19+, surface IgG+, kappa+, were karyotypically abnormal and showed clonal rearrangements in the immunoglobulin heavy (IgH) and kappa light chain genes. Phenotypically leukaemic cells and lymphoma cells at RS resembled CLL at diagnosis, but showed cytogenetic evolution. Geometrically leukaemic cells and lymphoma cells retained the initial clonal rearrangements in IGH and kappa genes, but showed additional supervening clonal rearrangements in both of these genes as the disease progressed to RS. Furthermore, the c-lambda DNA showed clonal rearrangements in the leukaemic cells and lymphoma cells at RS. This complete phenotypic and genotypic analysis of tumour cells during the course of the disease demonstrates the origin of lymphoma from CLL cells throngh progressive cytogenetic and molecular genetic changes in CLL cells.

Original languageEnglish (US)
Pages (from-to)613-616
Number of pages4
JournalBritish Journal of Haematology
Volume85
Issue number3
StatePublished - 1993

Fingerprint

B-Cell Chronic Lymphocytic Leukemia
Cytogenetics
Molecular Biology
Lymphoma
Immunoglobulin kappa-Chains
Genes
Meningitis
Longitudinal Studies
Immunoglobulin G

ASJC Scopus subject areas

  • Hematology

Cite this

Serial phenotypic, cytogenetic and molecular genetic studies in Richter's syndrome : Demonstration of lymphoma development from the chronic lymphocytic leukaemia cells. / Koduru, P. R K; Lichtman, S. M.; Smilari, T. F.; Sun, T.; Goh, J. C.; Karp, L.; Hall, W.; Hashimoto, S.; Chiorazzi, N.; Broome, J. D.

In: British Journal of Haematology, Vol. 85, No. 3, 1993, p. 613-616.

Research output: Contribution to journalArticle

Koduru, PRK, Lichtman, SM, Smilari, TF, Sun, T, Goh, JC, Karp, L, Hall, W, Hashimoto, S, Chiorazzi, N & Broome, JD 1993, 'Serial phenotypic, cytogenetic and molecular genetic studies in Richter's syndrome: Demonstration of lymphoma development from the chronic lymphocytic leukaemia cells', British Journal of Haematology, vol. 85, no. 3, pp. 613-616.
Koduru, P. R K ; Lichtman, S. M. ; Smilari, T. F. ; Sun, T. ; Goh, J. C. ; Karp, L. ; Hall, W. ; Hashimoto, S. ; Chiorazzi, N. ; Broome, J. D. / Serial phenotypic, cytogenetic and molecular genetic studies in Richter's syndrome : Demonstration of lymphoma development from the chronic lymphocytic leukaemia cells. In: British Journal of Haematology. 1993 ; Vol. 85, No. 3. pp. 613-616.
@article{fad38c1a54cb47b584e7730f1862a08b,
title = "Serial phenotypic, cytogenetic and molecular genetic studies in Richter's syndrome: Demonstration of lymphoma development from the chronic lymphocytic leukaemia cells",
abstract = "In this report we describe a unique longitudinal study on the clinical, phenotypic, cytogenetic and molecular genetic features of malignant cells from diagnosis of chronic lymphocytic leukaemia (CLL) to the development of lymphoma and lymphomatous meningitis. CLL cells at diagnosis were CD5+, CD19+, surface IgG+, kappa+, were karyotypically abnormal and showed clonal rearrangements in the immunoglobulin heavy (IgH) and kappa light chain genes. Phenotypically leukaemic cells and lymphoma cells at RS resembled CLL at diagnosis, but showed cytogenetic evolution. Geometrically leukaemic cells and lymphoma cells retained the initial clonal rearrangements in IGH and kappa genes, but showed additional supervening clonal rearrangements in both of these genes as the disease progressed to RS. Furthermore, the c-lambda DNA showed clonal rearrangements in the leukaemic cells and lymphoma cells at RS. This complete phenotypic and genotypic analysis of tumour cells during the course of the disease demonstrates the origin of lymphoma from CLL cells throngh progressive cytogenetic and molecular genetic changes in CLL cells.",
author = "Koduru, {P. R K} and Lichtman, {S. M.} and Smilari, {T. F.} and T. Sun and Goh, {J. C.} and L. Karp and W. Hall and S. Hashimoto and N. Chiorazzi and Broome, {J. D.}",
year = "1993",
language = "English (US)",
volume = "85",
pages = "613--616",
journal = "British Journal of Haematology",
issn = "0007-1048",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Serial phenotypic, cytogenetic and molecular genetic studies in Richter's syndrome

T2 - Demonstration of lymphoma development from the chronic lymphocytic leukaemia cells

AU - Koduru, P. R K

AU - Lichtman, S. M.

AU - Smilari, T. F.

AU - Sun, T.

AU - Goh, J. C.

AU - Karp, L.

AU - Hall, W.

AU - Hashimoto, S.

AU - Chiorazzi, N.

AU - Broome, J. D.

PY - 1993

Y1 - 1993

N2 - In this report we describe a unique longitudinal study on the clinical, phenotypic, cytogenetic and molecular genetic features of malignant cells from diagnosis of chronic lymphocytic leukaemia (CLL) to the development of lymphoma and lymphomatous meningitis. CLL cells at diagnosis were CD5+, CD19+, surface IgG+, kappa+, were karyotypically abnormal and showed clonal rearrangements in the immunoglobulin heavy (IgH) and kappa light chain genes. Phenotypically leukaemic cells and lymphoma cells at RS resembled CLL at diagnosis, but showed cytogenetic evolution. Geometrically leukaemic cells and lymphoma cells retained the initial clonal rearrangements in IGH and kappa genes, but showed additional supervening clonal rearrangements in both of these genes as the disease progressed to RS. Furthermore, the c-lambda DNA showed clonal rearrangements in the leukaemic cells and lymphoma cells at RS. This complete phenotypic and genotypic analysis of tumour cells during the course of the disease demonstrates the origin of lymphoma from CLL cells throngh progressive cytogenetic and molecular genetic changes in CLL cells.

AB - In this report we describe a unique longitudinal study on the clinical, phenotypic, cytogenetic and molecular genetic features of malignant cells from diagnosis of chronic lymphocytic leukaemia (CLL) to the development of lymphoma and lymphomatous meningitis. CLL cells at diagnosis were CD5+, CD19+, surface IgG+, kappa+, were karyotypically abnormal and showed clonal rearrangements in the immunoglobulin heavy (IgH) and kappa light chain genes. Phenotypically leukaemic cells and lymphoma cells at RS resembled CLL at diagnosis, but showed cytogenetic evolution. Geometrically leukaemic cells and lymphoma cells retained the initial clonal rearrangements in IGH and kappa genes, but showed additional supervening clonal rearrangements in both of these genes as the disease progressed to RS. Furthermore, the c-lambda DNA showed clonal rearrangements in the leukaemic cells and lymphoma cells at RS. This complete phenotypic and genotypic analysis of tumour cells during the course of the disease demonstrates the origin of lymphoma from CLL cells throngh progressive cytogenetic and molecular genetic changes in CLL cells.

UR - http://www.scopus.com/inward/record.url?scp=0027372034&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027372034&partnerID=8YFLogxK

M3 - Article

C2 - 8136284

AN - SCOPUS:0027372034

VL - 85

SP - 613

EP - 616

JO - British Journal of Haematology

JF - British Journal of Haematology

SN - 0007-1048

IS - 3

ER -