TY - JOUR
T1 - Seroepidemiology of human bocavirus defined using recombinant virus-like particles
AU - Kahn, Jeffrey
AU - Kesebir, Deniz
AU - Cotmore, Susan F.
AU - D'Abramo, Anthony
AU - Cosby, Christi
AU - Weibel, Carla
AU - Tattersall, Peter
N1 - Funding Information:
Received 23 October 2007; accepted 28 December 2007; electronically published 19 May 2008. Potential conflicts of interest: none reported. Presented in part: Pediatric Academic Societies/Society of Pediatric Research Annual Meeting, Toronto, 3–8 May 2007 (abstract 6295.2). Financial support: Public Health Service, National Institutes of Health (training grant T32HD 07094 to D.K. and grant AI 072166 to P.T.); MedImmune (pediatric fellowship grant to D.K.). Reprints or correspondence: Dr. Jeffrey S. Kahn, Dept. of Pediatrics, Div. of Infectious Diseases, Yale University School of Medicine, PO Box 208064, New Haven, CT 06520-8064 (jeffrey.kahn@yale.edu).
PY - 2008/7/1
Y1 - 2008/7/1
N2 - Background. Human bocavirus (HBoV) is a newly identified human parvovirus for which seroepidemiology and antigenic properties remain undefined. Methods. The HBoV VP2 gene, expressed from a baculovirus vector, produced virus-like particles (VLPs), which were used to raise rabbit anti-HBoV antisera and to develop an enzyme-linked immunosorbent assay (ELISA). The VLP-based ELISA was used to screen for HBoV-specific immunoglobulin G antibodies in a convenience sample of 270 serum specimens, mostly from children, obtained at Yale-New Haven Hospital; 208 specimens were also screened for erythrovirus B19-specific antibodies by a B19 VLP-based ELISA. Results. Immunofluorescence and ELISA showed that human parvoviruses HBoV and B19 are antigenically distinct. By the HBoV VLP-based ELISA, 91.8% and 63.6% of serum specimens from infants in the first and second months of life, respectively, were found to be seropositive, as were 45.4% from 3-month-old infants and 25.0% from 4-month-old infants. The percentages of HBoV-seropositive children increased to 40.7%-60.0% for children 5-47 months of age and to >85% for individuals ≥48 months old. However, the overall percentage of B19-seropositive individuals was <40.5% for all age groups screened. Conclusions. HBoV infection is common during childhood, but a minority of children and young adults screened have evidence of B19 infection.
AB - Background. Human bocavirus (HBoV) is a newly identified human parvovirus for which seroepidemiology and antigenic properties remain undefined. Methods. The HBoV VP2 gene, expressed from a baculovirus vector, produced virus-like particles (VLPs), which were used to raise rabbit anti-HBoV antisera and to develop an enzyme-linked immunosorbent assay (ELISA). The VLP-based ELISA was used to screen for HBoV-specific immunoglobulin G antibodies in a convenience sample of 270 serum specimens, mostly from children, obtained at Yale-New Haven Hospital; 208 specimens were also screened for erythrovirus B19-specific antibodies by a B19 VLP-based ELISA. Results. Immunofluorescence and ELISA showed that human parvoviruses HBoV and B19 are antigenically distinct. By the HBoV VLP-based ELISA, 91.8% and 63.6% of serum specimens from infants in the first and second months of life, respectively, were found to be seropositive, as were 45.4% from 3-month-old infants and 25.0% from 4-month-old infants. The percentages of HBoV-seropositive children increased to 40.7%-60.0% for children 5-47 months of age and to >85% for individuals ≥48 months old. However, the overall percentage of B19-seropositive individuals was <40.5% for all age groups screened. Conclusions. HBoV infection is common during childhood, but a minority of children and young adults screened have evidence of B19 infection.
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U2 - 10.1086/588674
DO - 10.1086/588674
M3 - Article
C2 - 18491974
AN - SCOPUS:46349104670
SN - 0022-1899
VL - 198
SP - 41
EP - 50
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 1
ER -