Seroepidemiology of strongyloidiasis in a thai village

Richard W. Douce; Arthur E. Brown; Chirasak Khamboonruang; Peter D. Walzer; Robert M. Genta

doi:10.1016/0020-7519(87)90101-9

Seroepidemiology of strongyloidiasis in a thai village

Richard W. Douce, Arthur E. Brown, Chirasak Khamboonruang, Peter D. Walzer, Robert M. Genta

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Strongyloidiasis is a potentially fatal disease for which existing methods of diagnosis lack sensitivity. A recently developed ELISA for IgG antibodies against the infective larval stage was used to screen a leprosy resettlement village in an area endemic for strongyloidiasis. The results of the ELISA were compared with the results of stool examination for 177 villagers. Eleven per cent of the village had S. stercoralis present on stool examination and 45% had antibodies detected by ELISA. Eighteen of 21 (85%) villagers with S. stercoralis infection proven by stool examination had positive ELISA values. No cross reactivity with intraluminal parasites was present. Linear correlation between per cent eosinophils and the ELISA revealed a correlation coefficient of 0.402 (P < 0.001). Forty-two days after sucessful treatment in 14 villagers, the IgG antibody levels had decreased (P < 0.001). Leprosy did not affect the sensitivity of the assay. These results suggest that ELISA is a simple, sensitive and specific method for surveying exposure to S. stercoralis in an endemic area.

Original language	English (US)
Pages (from-to)	1343-1348
Number of pages	6
Journal	International Journal for Parasitology
Volume	17
Issue number	7
DOIs	https://doi.org/10.1016/0020-7519(87)90101-9
State	Published - Oct 1987

Keywords

South East Asia
Strongyloides stercoralis
epidemiology
immunodiagnosis
serology
strongyloidiasis

ASJC Scopus subject areas

Parasitology
Infectious Diseases

Access to Document

10.1016/0020-7519(87)90101-9

Cite this

@article{279ed567821144a8bb81b2c5624b8407,

title = "Seroepidemiology of strongyloidiasis in a thai village",

abstract = "Strongyloidiasis is a potentially fatal disease for which existing methods of diagnosis lack sensitivity. A recently developed ELISA for IgG antibodies against the infective larval stage was used to screen a leprosy resettlement village in an area endemic for strongyloidiasis. The results of the ELISA were compared with the results of stool examination for 177 villagers. Eleven per cent of the village had S. stercoralis present on stool examination and 45% had antibodies detected by ELISA. Eighteen of 21 (85%) villagers with S. stercoralis infection proven by stool examination had positive ELISA values. No cross reactivity with intraluminal parasites was present. Linear correlation between per cent eosinophils and the ELISA revealed a correlation coefficient of 0.402 (P < 0.001). Forty-two days after sucessful treatment in 14 villagers, the IgG antibody levels had decreased (P < 0.001). Leprosy did not affect the sensitivity of the assay. These results suggest that ELISA is a simple, sensitive and specific method for surveying exposure to S. stercoralis in an endemic area.",

keywords = "South East Asia, Strongyloides stercoralis, epidemiology, immunodiagnosis, serology, strongyloidiasis",

author = "Douce, {Richard W.} and Brown, {Arthur E.} and Chirasak Khamboonruang and Walzer, {Peter D.} and Genta, {Robert M.}",

note = "Funding Information: Acknowledgements-Data analysis was completed with the assistance of the General Clinical Research Center, Clinfo RROO068. This research was supported in part by the Medical Research Service of the VA. Dr. Walzer is a clinical investigator with the VA. The skilled technical assistance of MissTheresaHuitgerandMs.LindaHayden,whoperformed the total IgE assays, is gratefully acknowledged. The Document Processing Area of the Department of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, competently typed this manuscript. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1987",

month = oct,

doi = "10.1016/0020-7519(87)90101-9",

language = "English (US)",

volume = "17",

pages = "1343--1348",

journal = "International Journal for Parasitology",

issn = "0020-7519",

publisher = "Elsevier Limited",

number = "7",

}

TY - JOUR

T1 - Seroepidemiology of strongyloidiasis in a thai village

AU - Douce, Richard W.

AU - Brown, Arthur E.

AU - Khamboonruang, Chirasak

AU - Walzer, Peter D.

AU - Genta, Robert M.

N1 - Funding Information: Acknowledgements-Data analysis was completed with the assistance of the General Clinical Research Center, Clinfo RROO068. This research was supported in part by the Medical Research Service of the VA. Dr. Walzer is a clinical investigator with the VA. The skilled technical assistance of MissTheresaHuitgerandMs.LindaHayden,whoperformed the total IgE assays, is gratefully acknowledged. The Document Processing Area of the Department of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, competently typed this manuscript. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1987/10

Y1 - 1987/10

N2 - Strongyloidiasis is a potentially fatal disease for which existing methods of diagnosis lack sensitivity. A recently developed ELISA for IgG antibodies against the infective larval stage was used to screen a leprosy resettlement village in an area endemic for strongyloidiasis. The results of the ELISA were compared with the results of stool examination for 177 villagers. Eleven per cent of the village had S. stercoralis present on stool examination and 45% had antibodies detected by ELISA. Eighteen of 21 (85%) villagers with S. stercoralis infection proven by stool examination had positive ELISA values. No cross reactivity with intraluminal parasites was present. Linear correlation between per cent eosinophils and the ELISA revealed a correlation coefficient of 0.402 (P < 0.001). Forty-two days after sucessful treatment in 14 villagers, the IgG antibody levels had decreased (P < 0.001). Leprosy did not affect the sensitivity of the assay. These results suggest that ELISA is a simple, sensitive and specific method for surveying exposure to S. stercoralis in an endemic area.

AB - Strongyloidiasis is a potentially fatal disease for which existing methods of diagnosis lack sensitivity. A recently developed ELISA for IgG antibodies against the infective larval stage was used to screen a leprosy resettlement village in an area endemic for strongyloidiasis. The results of the ELISA were compared with the results of stool examination for 177 villagers. Eleven per cent of the village had S. stercoralis present on stool examination and 45% had antibodies detected by ELISA. Eighteen of 21 (85%) villagers with S. stercoralis infection proven by stool examination had positive ELISA values. No cross reactivity with intraluminal parasites was present. Linear correlation between per cent eosinophils and the ELISA revealed a correlation coefficient of 0.402 (P < 0.001). Forty-two days after sucessful treatment in 14 villagers, the IgG antibody levels had decreased (P < 0.001). Leprosy did not affect the sensitivity of the assay. These results suggest that ELISA is a simple, sensitive and specific method for surveying exposure to S. stercoralis in an endemic area.

KW - South East Asia

KW - Strongyloides stercoralis

KW - epidemiology

KW - immunodiagnosis

KW - serology

KW - strongyloidiasis

UR - http://www.scopus.com/inward/record.url?scp=0023630806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023630806&partnerID=8YFLogxK

U2 - 10.1016/0020-7519(87)90101-9

DO - 10.1016/0020-7519(87)90101-9

M3 - Article

C2 - 3429125

AN - SCOPUS:0023630806

SN - 0020-7519

VL - 17

SP - 1343

EP - 1348

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 7

ER -

Seroepidemiology of strongyloidiasis in a thai village

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this