Serological antibodies against LY6K as a diagnostic biomarker in esophageal squamous cell carcinoma

Bin Zhang; Zhengliang Zhang; Xufei Zhang; Xu Gao; Kemp H. Kernstine; Li Zhong

doi:10.3109/1354750X.2012.680609

Serological antibodies against LY6K as a diagnostic biomarker in esophageal squamous cell carcinoma

Bin Zhang, Zhengliang Zhang, Xufei Zhang, Xu Gao, Kemp H. Kernstine, Li Zhong

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Objectives: To evaluate the diagnostic values of autoantibodies against lymphocyte antigen 6 complex locus K (LY6K) in esophageal squamous cell carcinoma (ESCC). Methods: After cloning, expressing, and purifying LY6K as fusion proteins, LY6K autoantibodies were measured in 62 patient and 58 control serum samples using enzyme-linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the LY6K mRNA levels in ESCC and adjacent tissues. Results: LY6K autoantibodies were found significantly higher in patients than controls. The area under the receiver-operating characteristic (ROC) curve (AUC) was 0.85, and the optimal sensitivity and specificity for ESCC detection were 80.6 and 78.7%, respectively. LY6K mRNA expressions in patients were upregulated. Conclusions: Autoantibodies against LY6K may be a good diagnostic biomarker for ESCC.

Original language	English (US)
Pages (from-to)	372-378
Number of pages	7
Journal	Biomarkers
Volume	17
Issue number	4
DOIs	https://doi.org/10.3109/1354750X.2012.680609
State	Published - Jun 2012

Keywords

Gastric cancer
Gene expression
Proteomics

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Health, Toxicology and Mutagenesis

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10.3109/1354750X.2012.680609

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@article{11fdbaed10c34712a3fb0ce25bc44345,

title = "Serological antibodies against LY6K as a diagnostic biomarker in esophageal squamous cell carcinoma",

abstract = "Objectives: To evaluate the diagnostic values of autoantibodies against lymphocyte antigen 6 complex locus K (LY6K) in esophageal squamous cell carcinoma (ESCC). Methods: After cloning, expressing, and purifying LY6K as fusion proteins, LY6K autoantibodies were measured in 62 patient and 58 control serum samples using enzyme-linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the LY6K mRNA levels in ESCC and adjacent tissues. Results: LY6K autoantibodies were found significantly higher in patients than controls. The area under the receiver-operating characteristic (ROC) curve (AUC) was 0.85, and the optimal sensitivity and specificity for ESCC detection were 80.6 and 78.7%, respectively. LY6K mRNA expressions in patients were upregulated. Conclusions: Autoantibodies against LY6K may be a good diagnostic biomarker for ESCC.",

keywords = "Gastric cancer, Gene expression, Proteomics",

author = "Bin Zhang and Zhengliang Zhang and Xufei Zhang and Xu Gao and Kernstine, {Kemp H.} and Li Zhong",

note = "Funding Information: This study is supported by NIH grant CA137570 (L Zhong) and NSFC Grant #81071795.",

year = "2012",

month = jun,

doi = "10.3109/1354750X.2012.680609",

language = "English (US)",

volume = "17",

pages = "372--378",

journal = "Biomarkers",

issn = "1354-750X",

publisher = "Informa Healthcare",

number = "4",

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TY - JOUR

T1 - Serological antibodies against LY6K as a diagnostic biomarker in esophageal squamous cell carcinoma

AU - Zhang, Bin

AU - Zhang, Zhengliang

AU - Zhang, Xufei

AU - Gao, Xu

AU - Kernstine, Kemp H.

AU - Zhong, Li

N1 - Funding Information: This study is supported by NIH grant CA137570 (L Zhong) and NSFC Grant #81071795.

PY - 2012/6

Y1 - 2012/6

N2 - Objectives: To evaluate the diagnostic values of autoantibodies against lymphocyte antigen 6 complex locus K (LY6K) in esophageal squamous cell carcinoma (ESCC). Methods: After cloning, expressing, and purifying LY6K as fusion proteins, LY6K autoantibodies were measured in 62 patient and 58 control serum samples using enzyme-linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the LY6K mRNA levels in ESCC and adjacent tissues. Results: LY6K autoantibodies were found significantly higher in patients than controls. The area under the receiver-operating characteristic (ROC) curve (AUC) was 0.85, and the optimal sensitivity and specificity for ESCC detection were 80.6 and 78.7%, respectively. LY6K mRNA expressions in patients were upregulated. Conclusions: Autoantibodies against LY6K may be a good diagnostic biomarker for ESCC.

AB - Objectives: To evaluate the diagnostic values of autoantibodies against lymphocyte antigen 6 complex locus K (LY6K) in esophageal squamous cell carcinoma (ESCC). Methods: After cloning, expressing, and purifying LY6K as fusion proteins, LY6K autoantibodies were measured in 62 patient and 58 control serum samples using enzyme-linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the LY6K mRNA levels in ESCC and adjacent tissues. Results: LY6K autoantibodies were found significantly higher in patients than controls. The area under the receiver-operating characteristic (ROC) curve (AUC) was 0.85, and the optimal sensitivity and specificity for ESCC detection were 80.6 and 78.7%, respectively. LY6K mRNA expressions in patients were upregulated. Conclusions: Autoantibodies against LY6K may be a good diagnostic biomarker for ESCC.

KW - Gastric cancer

KW - Gene expression

KW - Proteomics

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U2 - 10.3109/1354750X.2012.680609

DO - 10.3109/1354750X.2012.680609

M3 - Article

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AN - SCOPUS:84861625984

SN - 1354-750X

VL - 17

SP - 372

EP - 378

JO - Biomarkers

JF - Biomarkers

IS - 4

ER -

Serological antibodies against LY6K as a diagnostic biomarker in esophageal squamous cell carcinoma

Abstract

Keywords

ASJC Scopus subject areas

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