Serological antibodies against LY6K as a diagnostic biomarker in esophageal squamous cell carcinoma

Bin Zhang, Zhengliang Zhang, Xufei Zhang, Xu Gao, Kemp H. Kernstine, Li Zhong

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objectives: To evaluate the diagnostic values of autoantibodies against lymphocyte antigen 6 complex locus K (LY6K) in esophageal squamous cell carcinoma (ESCC). Methods: After cloning, expressing, and purifying LY6K as fusion proteins, LY6K autoantibodies were measured in 62 patient and 58 control serum samples using enzyme-linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the LY6K mRNA levels in ESCC and adjacent tissues. Results: LY6K autoantibodies were found significantly higher in patients than controls. The area under the receiver-operating characteristic (ROC) curve (AUC) was 0.85, and the optimal sensitivity and specificity for ESCC detection were 80.6 and 78.7%, respectively. LY6K mRNA expressions in patients were upregulated. Conclusions: Autoantibodies against LY6K may be a good diagnostic biomarker for ESCC.

Original languageEnglish (US)
Pages (from-to)372-378
Number of pages7
JournalBiomarkers
Volume17
Issue number4
DOIs
StatePublished - Jun 2012

Fingerprint

Biomarkers
Autoantibodies
Antibodies
Immunosorbents
Messenger RNA
Lymphocytes
Cloning
Polymerase chain reaction
Transcription
ROC Curve
Reverse Transcription
Area Under Curve
Organism Cloning
Assays
Fusion reactions
Enzyme-Linked Immunosorbent Assay
Tissue
Antigens
Sensitivity and Specificity
Polymerase Chain Reaction

Keywords

  • Gastric cancer
  • Gene expression
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Health, Toxicology and Mutagenesis

Cite this

Serological antibodies against LY6K as a diagnostic biomarker in esophageal squamous cell carcinoma. / Zhang, Bin; Zhang, Zhengliang; Zhang, Xufei; Gao, Xu; Kernstine, Kemp H.; Zhong, Li.

In: Biomarkers, Vol. 17, No. 4, 06.2012, p. 372-378.

Research output: Contribution to journalArticle

Zhang, Bin ; Zhang, Zhengliang ; Zhang, Xufei ; Gao, Xu ; Kernstine, Kemp H. ; Zhong, Li. / Serological antibodies against LY6K as a diagnostic biomarker in esophageal squamous cell carcinoma. In: Biomarkers. 2012 ; Vol. 17, No. 4. pp. 372-378.
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abstract = "Objectives: To evaluate the diagnostic values of autoantibodies against lymphocyte antigen 6 complex locus K (LY6K) in esophageal squamous cell carcinoma (ESCC). Methods: After cloning, expressing, and purifying LY6K as fusion proteins, LY6K autoantibodies were measured in 62 patient and 58 control serum samples using enzyme-linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the LY6K mRNA levels in ESCC and adjacent tissues. Results: LY6K autoantibodies were found significantly higher in patients than controls. The area under the receiver-operating characteristic (ROC) curve (AUC) was 0.85, and the optimal sensitivity and specificity for ESCC detection were 80.6 and 78.7{\%}, respectively. LY6K mRNA expressions in patients were upregulated. Conclusions: Autoantibodies against LY6K may be a good diagnostic biomarker for ESCC.",
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AU - Zhong, Li

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N2 - Objectives: To evaluate the diagnostic values of autoantibodies against lymphocyte antigen 6 complex locus K (LY6K) in esophageal squamous cell carcinoma (ESCC). Methods: After cloning, expressing, and purifying LY6K as fusion proteins, LY6K autoantibodies were measured in 62 patient and 58 control serum samples using enzyme-linked immunosorbent assay (ELISA). Reverse transcription polymerase chain reaction (RT-PCR) was used to measure the LY6K mRNA levels in ESCC and adjacent tissues. Results: LY6K autoantibodies were found significantly higher in patients than controls. The area under the receiver-operating characteristic (ROC) curve (AUC) was 0.85, and the optimal sensitivity and specificity for ESCC detection were 80.6 and 78.7%, respectively. LY6K mRNA expressions in patients were upregulated. Conclusions: Autoantibodies against LY6K may be a good diagnostic biomarker for ESCC.

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