SERPINB3 is associated with TGF-β1 and cytoplasmic β-catenin expression in hepatocellular carcinomas with poor prognosis

C. Turato, A. Vitale, S. Fasolato, M. Ruvoletto, L. Terrin, S. Quarta, R. Ramirez Morales, A. Biasiolo, G. Zanus, N. Zali, P. S. Tan, Y. Hoshida, A. Gatta, U. Cillo, P. Pontisso

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background:Hepatocellular carcinoma (HCC) is one of the most important sanitary problems for its prevalence and poor prognosis. To date, no information is available on the prognostic value of the ov-serpin SERPINB3, detected in primary liver cancer but not in normal liver. The aim of the study was to analyse SERPINB3 expression in liver cancer in relation with molecular signatures of poor prognosis and with clinical outcome.Methods:Liver tumours of 97 patients were analysed in parallel for SERPINB3, TGF-β and β-catenin. In a subgroup of 67 patients with adequate clinical follow-up, the correlation of molecular findings with clinical outcome was also carried out.Results:High SERPINB3 levels were detectable in 22% of the patients. A significant correlation of this serpin with TGF-β at transcription and protein level was observed, whereas for β-catenin a strong correlation was found only at post-transcription level. These findings were in agreement with transcriptome data meta-analysis, showing accumulation of SERPINB3 in the poor-prognosis subclass (S1). High levels of this serpin were significantly associated with early tumour recurrence and high SERPINB3 was the only variable significantly associated with time to recurrence at multivariate analysis.Conclusions:SERPINB3 is overexpressed in the subset of the most aggressive HCCs.

Original languageEnglish (US)
Pages (from-to)2708-2715
Number of pages8
JournalBritish Journal of Cancer
Volume110
Issue number11
DOIs
StatePublished - May 27 2014
Externally publishedYes

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Serpins
Catenins
Hepatocellular Carcinoma
Liver Neoplasms
Recurrence
Liver
Transcriptome
Meta-Analysis
Neoplasms
Multivariate Analysis
Proteins

Keywords

  • b-catenin
  • hepatocellular carcinoma subclasses
  • SERPINB3
  • TGF-b
  • tumour recurrence

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Turato, C., Vitale, A., Fasolato, S., Ruvoletto, M., Terrin, L., Quarta, S., ... Pontisso, P. (2014). SERPINB3 is associated with TGF-β1 and cytoplasmic β-catenin expression in hepatocellular carcinomas with poor prognosis. British Journal of Cancer, 110(11), 2708-2715. https://doi.org/10.1038/bjc.2014.246

SERPINB3 is associated with TGF-β1 and cytoplasmic β-catenin expression in hepatocellular carcinomas with poor prognosis. / Turato, C.; Vitale, A.; Fasolato, S.; Ruvoletto, M.; Terrin, L.; Quarta, S.; Ramirez Morales, R.; Biasiolo, A.; Zanus, G.; Zali, N.; Tan, P. S.; Hoshida, Y.; Gatta, A.; Cillo, U.; Pontisso, P.

In: British Journal of Cancer, Vol. 110, No. 11, 27.05.2014, p. 2708-2715.

Research output: Contribution to journalArticle

Turato, C, Vitale, A, Fasolato, S, Ruvoletto, M, Terrin, L, Quarta, S, Ramirez Morales, R, Biasiolo, A, Zanus, G, Zali, N, Tan, PS, Hoshida, Y, Gatta, A, Cillo, U & Pontisso, P 2014, 'SERPINB3 is associated with TGF-β1 and cytoplasmic β-catenin expression in hepatocellular carcinomas with poor prognosis', British Journal of Cancer, vol. 110, no. 11, pp. 2708-2715. https://doi.org/10.1038/bjc.2014.246
Turato, C. ; Vitale, A. ; Fasolato, S. ; Ruvoletto, M. ; Terrin, L. ; Quarta, S. ; Ramirez Morales, R. ; Biasiolo, A. ; Zanus, G. ; Zali, N. ; Tan, P. S. ; Hoshida, Y. ; Gatta, A. ; Cillo, U. ; Pontisso, P. / SERPINB3 is associated with TGF-β1 and cytoplasmic β-catenin expression in hepatocellular carcinomas with poor prognosis. In: British Journal of Cancer. 2014 ; Vol. 110, No. 11. pp. 2708-2715.
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abstract = "Background:Hepatocellular carcinoma (HCC) is one of the most important sanitary problems for its prevalence and poor prognosis. To date, no information is available on the prognostic value of the ov-serpin SERPINB3, detected in primary liver cancer but not in normal liver. The aim of the study was to analyse SERPINB3 expression in liver cancer in relation with molecular signatures of poor prognosis and with clinical outcome.Methods:Liver tumours of 97 patients were analysed in parallel for SERPINB3, TGF-β and β-catenin. In a subgroup of 67 patients with adequate clinical follow-up, the correlation of molecular findings with clinical outcome was also carried out.Results:High SERPINB3 levels were detectable in 22{\%} of the patients. A significant correlation of this serpin with TGF-β at transcription and protein level was observed, whereas for β-catenin a strong correlation was found only at post-transcription level. These findings were in agreement with transcriptome data meta-analysis, showing accumulation of SERPINB3 in the poor-prognosis subclass (S1). High levels of this serpin were significantly associated with early tumour recurrence and high SERPINB3 was the only variable significantly associated with time to recurrence at multivariate analysis.Conclusions:SERPINB3 is overexpressed in the subset of the most aggressive HCCs.",
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T1 - SERPINB3 is associated with TGF-β1 and cytoplasmic β-catenin expression in hepatocellular carcinomas with poor prognosis

AU - Turato, C.

AU - Vitale, A.

AU - Fasolato, S.

AU - Ruvoletto, M.

AU - Terrin, L.

AU - Quarta, S.

AU - Ramirez Morales, R.

AU - Biasiolo, A.

AU - Zanus, G.

AU - Zali, N.

AU - Tan, P. S.

AU - Hoshida, Y.

AU - Gatta, A.

AU - Cillo, U.

AU - Pontisso, P.

PY - 2014/5/27

Y1 - 2014/5/27

N2 - Background:Hepatocellular carcinoma (HCC) is one of the most important sanitary problems for its prevalence and poor prognosis. To date, no information is available on the prognostic value of the ov-serpin SERPINB3, detected in primary liver cancer but not in normal liver. The aim of the study was to analyse SERPINB3 expression in liver cancer in relation with molecular signatures of poor prognosis and with clinical outcome.Methods:Liver tumours of 97 patients were analysed in parallel for SERPINB3, TGF-β and β-catenin. In a subgroup of 67 patients with adequate clinical follow-up, the correlation of molecular findings with clinical outcome was also carried out.Results:High SERPINB3 levels were detectable in 22% of the patients. A significant correlation of this serpin with TGF-β at transcription and protein level was observed, whereas for β-catenin a strong correlation was found only at post-transcription level. These findings were in agreement with transcriptome data meta-analysis, showing accumulation of SERPINB3 in the poor-prognosis subclass (S1). High levels of this serpin were significantly associated with early tumour recurrence and high SERPINB3 was the only variable significantly associated with time to recurrence at multivariate analysis.Conclusions:SERPINB3 is overexpressed in the subset of the most aggressive HCCs.

AB - Background:Hepatocellular carcinoma (HCC) is one of the most important sanitary problems for its prevalence and poor prognosis. To date, no information is available on the prognostic value of the ov-serpin SERPINB3, detected in primary liver cancer but not in normal liver. The aim of the study was to analyse SERPINB3 expression in liver cancer in relation with molecular signatures of poor prognosis and with clinical outcome.Methods:Liver tumours of 97 patients were analysed in parallel for SERPINB3, TGF-β and β-catenin. In a subgroup of 67 patients with adequate clinical follow-up, the correlation of molecular findings with clinical outcome was also carried out.Results:High SERPINB3 levels were detectable in 22% of the patients. A significant correlation of this serpin with TGF-β at transcription and protein level was observed, whereas for β-catenin a strong correlation was found only at post-transcription level. These findings were in agreement with transcriptome data meta-analysis, showing accumulation of SERPINB3 in the poor-prognosis subclass (S1). High levels of this serpin were significantly associated with early tumour recurrence and high SERPINB3 was the only variable significantly associated with time to recurrence at multivariate analysis.Conclusions:SERPINB3 is overexpressed in the subset of the most aggressive HCCs.

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