Serum Amyloid A3 Gene Expression in Adipocytes is an Indicator of the Interaction with Macrophages

Yohei Sanada, Takafumi Yamamoto, Rika Satake, Akiko Yamashita, Sumire Kanai, Norihisa Kato, Fons Aj Van De Loo, Fusanori Nishimura, Philipp E. Scherer, Noriyuki Yanaka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The infiltration of macrophages into adipose tissue and their interaction with adipocytes are essential for the chronic low-grade inflammation of obese adipose tissue. In this study, we identified the serum amyloid A3 (Saa3) gene as a key adipocyte-derived factor that is affected by interaction with macrophages. We showed that the Saa3 promoter in adipocytes actually responds to activated macrophages in a co-culture system. Decreasing C/EBPβ abundance in 3T3-L1 adipocytes or point mutation of C/EBPβ elements suppressed the increased promoter activity in response to activated macrophages, suggesting an essential role of C/EBPβ in Saa3 promoter activation. Bioluminescence based on Saa3 promoter activity in Saa3-luc mice was promoted in obese adipose tissue, showing that Saa3 promoter activity is most likely related to macrophage infiltration. This study suggests that the level of expression of the Saa3 gene could be utilized for the number of infiltrated macrophages in obese adipose tissue.

Original languageEnglish (US)
Article number38697
JournalScientific Reports
Volume6
DOIs
StatePublished - Dec 8 2016

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Amyloid
Adipocytes
Macrophages
Gene Expression
Serum
Adipose Tissue
Coculture Techniques
Point Mutation
Genes
Inflammation

ASJC Scopus subject areas

  • General

Cite this

Sanada, Y., Yamamoto, T., Satake, R., Yamashita, A., Kanai, S., Kato, N., ... Yanaka, N. (2016). Serum Amyloid A3 Gene Expression in Adipocytes is an Indicator of the Interaction with Macrophages. Scientific Reports, 6, [38697]. https://doi.org/10.1038/srep38697

Serum Amyloid A3 Gene Expression in Adipocytes is an Indicator of the Interaction with Macrophages. / Sanada, Yohei; Yamamoto, Takafumi; Satake, Rika; Yamashita, Akiko; Kanai, Sumire; Kato, Norihisa; Van De Loo, Fons Aj; Nishimura, Fusanori; Scherer, Philipp E.; Yanaka, Noriyuki.

In: Scientific Reports, Vol. 6, 38697, 08.12.2016.

Research output: Contribution to journalArticle

Sanada, Y, Yamamoto, T, Satake, R, Yamashita, A, Kanai, S, Kato, N, Van De Loo, FA, Nishimura, F, Scherer, PE & Yanaka, N 2016, 'Serum Amyloid A3 Gene Expression in Adipocytes is an Indicator of the Interaction with Macrophages', Scientific Reports, vol. 6, 38697. https://doi.org/10.1038/srep38697
Sanada, Yohei ; Yamamoto, Takafumi ; Satake, Rika ; Yamashita, Akiko ; Kanai, Sumire ; Kato, Norihisa ; Van De Loo, Fons Aj ; Nishimura, Fusanori ; Scherer, Philipp E. ; Yanaka, Noriyuki. / Serum Amyloid A3 Gene Expression in Adipocytes is an Indicator of the Interaction with Macrophages. In: Scientific Reports. 2016 ; Vol. 6.
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