Serum autoantibody against glutathione S-transferase in patients with glaucoma

J. Yang, G. Tezel, R. V. Patil, C. Romano, M. B. Wax

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Purpose. To identify retinal proteins that are the targets of serum autoantibodies in patients with glaucoma. Methods. To identify retinal antigens that are recognized by the sera of patients with glaucoma, immunoreactive bands were separated, by using two-dimensional gel electrophoresis of the bovine retinal soluble fraction. A 29-kDa band was then selected for further analysis. Tryptic peptides of the 29-kDa band were analyzed using electrospray mass spectrometry, to identify the protein. After protein identification, immunoreactivity against this newly identified protein was studied by Western blot analysis using sera from 65 patients with glaucoma (25 with primary open-angle glaucoma [POAG], 40 with normal-pressure glaucoma [NPG]) and 25 age-matched healthy subjects. In addition, serum antibody titers were compared in these groups, by using a specific enzyme-linked immunosorbent assay (ELISA). Results. The 29-kDa band was identified as glutathione S-transferase (GST). Western blot analysis revealed that serum antibodies against GST antigen were recognized in 34 (52%) of 65 patients with glaucoma (22 of NPG and 12 of POAG) and 5 (20%) of 25 age-matched control subjects (X2 test, P < 0.05). By ELISA, it was also found that patients with glaucoma had higher titers of anti-GST antibody, compared with the control group (Mann-Whitney test, NPG versus control, P = 0.013; POAG versus control, P = 0.0006). Conclusions. These findings indicate that GST is one of the retinal antigens targeted by the serum antibodies detected in some patients with glaucoma.

Original languageEnglish (US)
Pages (from-to)1273-1276
Number of pages4
JournalInvestigative Ophthalmology and Visual Science
Volume42
Issue number6
StatePublished - 2001

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Glutathione Transferase
Glaucoma
Autoantibodies
Serum
Antibodies
Antigens
Pressure
Western Blotting
Enzyme-Linked Immunosorbent Assay
Proteins
Electrophoresis, Gel, Two-Dimensional
Blood Proteins
Mass Spectrometry
Healthy Volunteers
Control Groups
Peptides

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Yang, J., Tezel, G., Patil, R. V., Romano, C., & Wax, M. B. (2001). Serum autoantibody against glutathione S-transferase in patients with glaucoma. Investigative Ophthalmology and Visual Science, 42(6), 1273-1276.

Serum autoantibody against glutathione S-transferase in patients with glaucoma. / Yang, J.; Tezel, G.; Patil, R. V.; Romano, C.; Wax, M. B.

In: Investigative Ophthalmology and Visual Science, Vol. 42, No. 6, 2001, p. 1273-1276.

Research output: Contribution to journalArticle

Yang, J, Tezel, G, Patil, RV, Romano, C & Wax, MB 2001, 'Serum autoantibody against glutathione S-transferase in patients with glaucoma', Investigative Ophthalmology and Visual Science, vol. 42, no. 6, pp. 1273-1276.
Yang, J. ; Tezel, G. ; Patil, R. V. ; Romano, C. ; Wax, M. B. / Serum autoantibody against glutathione S-transferase in patients with glaucoma. In: Investigative Ophthalmology and Visual Science. 2001 ; Vol. 42, No. 6. pp. 1273-1276.
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abstract = "Purpose. To identify retinal proteins that are the targets of serum autoantibodies in patients with glaucoma. Methods. To identify retinal antigens that are recognized by the sera of patients with glaucoma, immunoreactive bands were separated, by using two-dimensional gel electrophoresis of the bovine retinal soluble fraction. A 29-kDa band was then selected for further analysis. Tryptic peptides of the 29-kDa band were analyzed using electrospray mass spectrometry, to identify the protein. After protein identification, immunoreactivity against this newly identified protein was studied by Western blot analysis using sera from 65 patients with glaucoma (25 with primary open-angle glaucoma [POAG], 40 with normal-pressure glaucoma [NPG]) and 25 age-matched healthy subjects. In addition, serum antibody titers were compared in these groups, by using a specific enzyme-linked immunosorbent assay (ELISA). Results. The 29-kDa band was identified as glutathione S-transferase (GST). Western blot analysis revealed that serum antibodies against GST antigen were recognized in 34 (52{\%}) of 65 patients with glaucoma (22 of NPG and 12 of POAG) and 5 (20{\%}) of 25 age-matched control subjects (X2 test, P < 0.05). By ELISA, it was also found that patients with glaucoma had higher titers of anti-GST antibody, compared with the control group (Mann-Whitney test, NPG versus control, P = 0.013; POAG versus control, P = 0.0006). Conclusions. These findings indicate that GST is one of the retinal antigens targeted by the serum antibodies detected in some patients with glaucoma.",
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