Severe muscle wasting and denervation in mice lacking the RNA-binding protein ZFP106

Douglas M. Anderson, Jessica Cannavino, Hui Li, Kelly M. Anderson, Benjamin R. Nelson, John McAnally, Svetlana Bezprozvannaya, Yun Liu, Weichun Lin, Ning Liu, Rhonda Bassel-Duby, Eric N. Olson

Research output: Contribution to journalArticle

Abstract

Innervation of skeletal muscle by motor neurons occurs through the neuromuscular junction, a cholinergic synapse essential for normal muscle growth and function. Defects in nerve-muscle signaling cause a variety of neuromuscular disorders with features of ataxia, paralysis, skeletal muscle wasting, and degeneration. Here we show that the nuclear zinc finger protein ZFP106 is highly enriched in skeletal muscle and is required for postnatal maintenance of myofiber innervation by motor neurons. Genetic disruption of Zfp106 in mice results in progressive ataxia and hindlimb paralysis associated with motor neuron degeneration, severe muscle wasting, and premature death by 6 mo of age.We show that ZFP106 is an RNA-binding protein that associates with the core splicing factor RNA binding motif protein 39 (RBM39) and localizes to nuclear speckles adjacent to spliceosomes. Upon inhibition of pre-mRNA synthesis, ZFP106 translocates with other splicing factors to the nucleolus. Muscle and spinal cord of Zfp106 knockout mice displayed a gene expression signature of neuromuscular degeneration. Strikingly, altered splicing of the Nogo (Rtn4) gene locus in skeletal muscle of Zfp106 knockout mice resulted in ectopic expression of NOGO-A, the neurite outgrowth factor that inhibits nerve regeneration and destabilizes neuromuscular junctions. These findings reveal a central role for Zfp106 in the maintenance of nerve-muscle signaling, and highlight the involvement of aberrant RNA processing in neuromuscular disease pathogenesis.

Original languageEnglish (US)
Pages (from-to)E4494-E4503
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number31
DOIs
StatePublished - Aug 2 2016

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Muscle Denervation
RNA-Binding Proteins
Skeletal Muscle
Motor Neurons
Muscles
Neuromuscular Junction
Ataxia
Knockout Mice
Paralysis
Maintenance
Spliceosomes
Nerve Degeneration
Neuromuscular Diseases
Nerve Regeneration
Premature Mortality
Zinc Fingers
RNA Precursors
Nerve Growth Factors
Hindlimb
Transcriptome

ASJC Scopus subject areas

  • General

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Severe muscle wasting and denervation in mice lacking the RNA-binding protein ZFP106. / Anderson, Douglas M.; Cannavino, Jessica; Li, Hui; Anderson, Kelly M.; Nelson, Benjamin R.; McAnally, John; Bezprozvannaya, Svetlana; Liu, Yun; Lin, Weichun; Liu, Ning; Bassel-Duby, Rhonda; Olson, Eric N.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 31, 02.08.2016, p. E4494-E4503.

Research output: Contribution to journalArticle

Anderson, Douglas M. ; Cannavino, Jessica ; Li, Hui ; Anderson, Kelly M. ; Nelson, Benjamin R. ; McAnally, John ; Bezprozvannaya, Svetlana ; Liu, Yun ; Lin, Weichun ; Liu, Ning ; Bassel-Duby, Rhonda ; Olson, Eric N. / Severe muscle wasting and denervation in mice lacking the RNA-binding protein ZFP106. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 31. pp. E4494-E4503.
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