Abstract
The aim of our study was to measure globotriaosylceramide (Gb3) and lyso-Gb3 levels by tandem mass spectrometry in the urine and kidney in Fabry (gla knockout) mice and wild-type controls. We found that urine Gb3 of male and female Fabry mice was higher than wild-type mice of the same sex but also significantly higher in male mice compared with females of the same genotype. In kidney tissue, sex and genotype-dependent differences in Gb3levels paralleled those in the urine. Isoforms C16, C22:1, and C24OHA were particularly higher in males compared with females in both wild-type and Fabry mice. Similarly, kidney lyso-Gb3 concentrations were significantly higher in 12-month-old male Fabry mice than in their homozygous female counterparts. However, lyso-Gb 3 was undetectable in wild-type mice of both sexes. α-Galactosidase A activity and mRNA levels in kidney were significantly lower in male wild-type mice compared with female mice. This study shows the sex differences in kidney and urine Gb3 and kidney lyso-Gb3 levels in both wild-type and Fabry mice, and it suggests that these male-female differences should be taken into consideration when using murine models for Fabry disease.
Original language | English (US) |
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Pages (from-to) | 1742-1746 |
Number of pages | 5 |
Journal | Journal of lipid research |
Volume | 52 |
Issue number | 9 |
DOIs | |
State | Published - Sep 2011 |
Externally published | Yes |
Keywords
- Glycosphingolipids
- Mass spectrometry
- Testosterone
- Urine
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Cell Biology