Sex differences of urinary and kidney globotriaosylceramide and lyso-globotriaosylceramide in fabry mice

Brandon Durant, Sabrina Forni, Lawrence Sweetman, Nastry Brignol, Xing Li Meng, Elfrida R. Benjamin, Raphael Schiffmann, Jin Song Shen

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of our study was to measure globotriaosylceramide (Gb3) and lyso-Gb3 levels by tandem mass spectrometry in the urine and kidney in Fabry (gla knockout) mice and wild-type controls. We found that urine Gb3 of male and female Fabry mice was higher than wild-type mice of the same sex but also significantly higher in male mice compared with females of the same genotype. In kidney tissue, sex and genotype-dependent differences in Gb3levels paralleled those in the urine. Isoforms C16, C22:1, and C24OHA were particularly higher in males compared with females in both wild-type and Fabry mice. Similarly, kidney lyso-Gb3 concentrations were significantly higher in 12-month-old male Fabry mice than in their homozygous female counterparts. However, lyso-Gb 3 was undetectable in wild-type mice of both sexes. α-Galactosidase A activity and mRNA levels in kidney were significantly lower in male wild-type mice compared with female mice. This study shows the sex differences in kidney and urine Gb3 and kidney lyso-Gb3 levels in both wild-type and Fabry mice, and it suggests that these male-female differences should be taken into consideration when using murine models for Fabry disease.

Original languageEnglish (US)
Pages (from-to)1742-1746
Number of pages5
JournalJournal of lipid research
Volume52
Issue number9
DOIs
StatePublished - Sep 2011

Keywords

  • Glycosphingolipids
  • Mass spectrometry
  • Testosterone
  • Urine

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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