SGEF enhances EGFR stability through delayed EGFR trafficking from early to late endosomes

Hongtao Wang, Shanhu Li, Hailiang Li, Changling Li, Kaopeng Guan, Guolan Luo, Lan Yu, Ruiqin Wu, Xiaoqing Zhang, Jian Wang, Jianguang Zhou

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Previously, we demonstrated an elevated SH3-containing guanine nucleotide exchange factor (SGEF) expression in clinical specimens with prostate cancer and implicated the role of SGEF in prostate tumorigenesis. However, the molecular mechanism behind the SGEF regulation of prostate cancer development is not known. In this study, we show that SGEF expression delays epidermal growth factor receptor (EGFR) degradation in prostate cancer cells and is independent from its guanine nucleotide exchange factor (GEF) function. We further show that the delayed degradation is due to a delay in EGFR trafficking from early to late endosomes and not to a decrease in EGFR ubiquitination. Finally, we show that depletion of SGEF significantly inhibits epidermal growth factor-induced EGFR signaling cascade and cell migration in the prostate cancer cells. We report for the first time an SGEF function for RhoG that excludes GEF and the ability of SGEF to enhance EGFR stability and signaling by delaying its lysosomal sorting and degradation. This could be one mechanism by which SGEF contributes to prostate cancer progression.

Original languageEnglish (US)
Pages (from-to)1976-1983
Number of pages8
JournalCarcinogenesis
Volume34
Issue number9
DOIs
StatePublished - Sep 1 2013
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research

Fingerprint Dive into the research topics of 'SGEF enhances EGFR stability through delayed EGFR trafficking from early to late endosomes'. Together they form a unique fingerprint.

  • Cite this

    Wang, H., Li, S., Li, H., Li, C., Guan, K., Luo, G., Yu, L., Wu, R., Zhang, X., Wang, J., & Zhou, J. (2013). SGEF enhances EGFR stability through delayed EGFR trafficking from early to late endosomes. Carcinogenesis, 34(9), 1976-1983. https://doi.org/10.1093/carcin/bgt157