SGNP

An essential stress granule/nucleolar protein potentially involved in 5.8s rRNA processing/transport

Chun Hong Zhu, Jinyong Kim, Jerry W. Shay, Woodring E. Wright

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Stress Granules (SG) are sites of accumulation of stalled initiation complexes that are induced following a variety of cellular insults. In a genetic screen for factors involved in protecting human myoblasts from acute oxidative stress, we identified a gene encoding a protein we designate SGNP (Stress Granule and Nucleolar Protein). Methodology/Principal Findings: A gene-trap insertional mutagenesis screen produced one insertion that conferred resistance to sodium arsenite. RT-PCR/3′ RACE was used to identify the endogenous gene expressed as a GFP-fusion transcript. SGNP is localized in both the cytoplasm and nucleolus and defines a non-nucleolar compartment containing 5.8S rRNA, a component of the 60S ribosomal subunit. Under oxidative stress, SGNP nucleolar localization decreases and it rapidly co-localizes with stress granules. The decrease in nucleolar SGNP following oxidative stress was accompanied by a large increase in nucleolar 5.8S rRNA. Knockdown of SGNP with shRNA increased global mRNA translation but induced growth arrest and cell death. Conclusions: These results suggest that SGNP is an essential gene that may be involved in ribosomal biogenesis and translational control in response to oxidative stress.

Original languageEnglish (US)
Article numbere3716
JournalPLoS One
Volume3
Issue number11
DOIs
StatePublished - Nov 13 2008

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Nuclear Proteins
Heat-Shock Proteins
granules
ribosomal RNA
Oxidative stress
Oxidative Stress
Processing
oxidative stress
proteins
Genes
Eukaryotic Large Ribosome Subunits
Myoblasts
Insertional Mutagenesis
Essential Genes
sodium arsenite
Protein Biosynthesis
Mutagenesis
Gene encoding
insertional mutagenesis
Small Interfering RNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

SGNP : An essential stress granule/nucleolar protein potentially involved in 5.8s rRNA processing/transport. / Zhu, Chun Hong; Kim, Jinyong; Shay, Jerry W.; Wright, Woodring E.

In: PLoS One, Vol. 3, No. 11, e3716, 13.11.2008.

Research output: Contribution to journalArticle

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