Shar-pei mediates cell proliferation arrest during imaginal disc growth in Drosophila

Madhuri Kango-Singh, Riitta Nolo, Chunyao Tao, Patrik Verstreken, P. Robin Hiesinger, Hugo J. Bellen, Georg Halder

Research output: Contribution to journalArticle

221 Citations (Scopus)

Abstract

During animal development, organ size is determined primarily by the amount of cell proliferation, which must be tightly regulated to ensure the generation of properly proportioned organs. However, little is known about the molecular pathways that direct cells to stop proliferating when an organ has attained its proper size. We have identified mutations in a novel gene, shar-pei, that is required for proper termination of cell proliferafion during Drosophild imaginal disc development. Clones of shar-pei mutant cells in imaginal discs produce enlarged tissues containing more cells of normal size. We show that this phenotype is the result of both increased cell proliferation and reduced apoptosis. Hence, shar-pei restricts cell proliferation and promotes apoptosis. By contrast, shar-pei is not required for cell differentiation and pattern formation of adult tissue. Shar-pei is also not required for cell cycle exit during terminal differentiation, indicating that the mechanisms directing cell proliferation arrest during organ growth are distinct from those directing cell cycle exit during terminal differentiation. shar-pei encodes a WW-domain-containing protein that has homologs in worms, mice and humans, suggesting that mechanisms of organ growth control are evolutionarily conserved.

Original languageEnglish (US)
Pages (from-to)5719-5730
Number of pages12
JournalDevelopment
Volume129
Issue number24
DOIs
StatePublished - Dec 2002

Fingerprint

Imaginal Discs
Drosophila
Cell Proliferation
Growth
Cell Cycle
Apoptosis
Organ Size
Cell Size
Cell Differentiation
Clone Cells
Phenotype
Mutation
Genes

Keywords

  • Apoptosis
  • Cell proliferation
  • Drosophila
  • Imaginal discs
  • WW domain-protein

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Kango-Singh, M., Nolo, R., Tao, C., Verstreken, P., Hiesinger, P. R., Bellen, H. J., & Halder, G. (2002). Shar-pei mediates cell proliferation arrest during imaginal disc growth in Drosophila. Development, 129(24), 5719-5730. https://doi.org/10.1242/dev.00168

Shar-pei mediates cell proliferation arrest during imaginal disc growth in Drosophila. / Kango-Singh, Madhuri; Nolo, Riitta; Tao, Chunyao; Verstreken, Patrik; Hiesinger, P. Robin; Bellen, Hugo J.; Halder, Georg.

In: Development, Vol. 129, No. 24, 12.2002, p. 5719-5730.

Research output: Contribution to journalArticle

Kango-Singh, M, Nolo, R, Tao, C, Verstreken, P, Hiesinger, PR, Bellen, HJ & Halder, G 2002, 'Shar-pei mediates cell proliferation arrest during imaginal disc growth in Drosophila', Development, vol. 129, no. 24, pp. 5719-5730. https://doi.org/10.1242/dev.00168
Kango-Singh M, Nolo R, Tao C, Verstreken P, Hiesinger PR, Bellen HJ et al. Shar-pei mediates cell proliferation arrest during imaginal disc growth in Drosophila. Development. 2002 Dec;129(24):5719-5730. https://doi.org/10.1242/dev.00168
Kango-Singh, Madhuri ; Nolo, Riitta ; Tao, Chunyao ; Verstreken, Patrik ; Hiesinger, P. Robin ; Bellen, Hugo J. ; Halder, Georg. / Shar-pei mediates cell proliferation arrest during imaginal disc growth in Drosophila. In: Development. 2002 ; Vol. 129, No. 24. pp. 5719-5730.
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