TY - JOUR
T1 - Sharp boundaries demarcate the chromatin structure of a yeast heat-shock gene
AU - Szent-Györgyi, Christopher
AU - Finkelstein, David B.
AU - Garrard, William T.
N1 - Funding Information:
‘Il’r arr grat.eful t,o V. c’. Hlasquez, I’. 5. Cockerill, I>. S. Gross. I). ~IcDaniels-(:erwig, S. M. Rose, and M. Xu for rrvit,w. and to M. Rotondi for preparation of the manuscript. C’.S.(:. was the recipient of a National Institutes of Health postdoctoral fellowship. This work was supported by grards GM22201, GM29935, GM25829. and GM3168!J from the X‘TH and grant, T-823 from the Robert. A. IV2’rl~hF oundation.
PY - 1987/1/5
Y1 - 1987/1/5
N2 - In both induced and basally transcribed states, the chromatin structure of the yeast HSP82 heat-shock locus exhibits a remarkable degree of organization with respect to DNA sequence. The promoter region contains a constitutive DNase I hypersensitive site. The transcription unit is markedly sensitive to DNase I, and exhibits a sharp transition from a phased half- to a whole nucleosomal cleavage periodicity at the 3′ end. Distant upstream and downstream regions are also organized into distinct arrays of phased nucleosomes. Each array is demarcated by DNase I hypersensitive sites that display internal protected regions, suggesting the presence of DNA binding proteins. In addition, since these sites are of mononucleosomal DNA length, they may acquire a nucleosomal structure under certain environmental conditions without disrupting flanking nucleosomal phasing frames. Thus, the HSP82 locus is organized into specific, phased, chromatin structures that appear to function in transcriptional initiation, RNA polymerase passage, transcriptional termination, and the establishment of chromatin-domain microenvironments.
AB - In both induced and basally transcribed states, the chromatin structure of the yeast HSP82 heat-shock locus exhibits a remarkable degree of organization with respect to DNA sequence. The promoter region contains a constitutive DNase I hypersensitive site. The transcription unit is markedly sensitive to DNase I, and exhibits a sharp transition from a phased half- to a whole nucleosomal cleavage periodicity at the 3′ end. Distant upstream and downstream regions are also organized into distinct arrays of phased nucleosomes. Each array is demarcated by DNase I hypersensitive sites that display internal protected regions, suggesting the presence of DNA binding proteins. In addition, since these sites are of mononucleosomal DNA length, they may acquire a nucleosomal structure under certain environmental conditions without disrupting flanking nucleosomal phasing frames. Thus, the HSP82 locus is organized into specific, phased, chromatin structures that appear to function in transcriptional initiation, RNA polymerase passage, transcriptional termination, and the establishment of chromatin-domain microenvironments.
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U2 - 10.1016/0022-2836(87)90628-0
DO - 10.1016/0022-2836(87)90628-0
M3 - Article
C2 - 3295258
AN - SCOPUS:0023089385
SN - 0022-2836
VL - 193
SP - 71
EP - 80
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 1
ER -