Shear stress-induced c-fos promoter activation is regulated by Rho GTPases and intracellular calcium

Yang Ting Elizabeth Shiu, Song Li, Yingxiao Wang, Suli Yuan, Benjamin Chen, Shu Chien

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Abstract

In this study, the molecular basis of c-fos promoter activation in endothelial cells (ECs) was explored in response to shear stress. Bovine aortic ECs(BAECs) were co-transfected with plasmids that encode a luciferease reporter gene controlled by c-fos promoter and the dominant-negative mutants of Rho, Cdc42, or Rac. Pre-treatment of BAECs with genistein, cytochalasin, or colchicine did not affect the shear induction of the c-fos promoter activity. It was found that shear-induced c-fos promoter activation is regulated by Rho and calcium, but is independent of Rac, Cdc42, cytoskeleton, and tyrosine kinase pathways.

Original languageEnglish (US)
JournalAnnals of Biomedical Engineering
Volume28
Issue numberSUPPL. 1
StatePublished - 2000

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ASJC Scopus subject areas

  • Biomedical Engineering

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