In this study, the molecular basis of c-fos promoter activation in endothelial cells (ECs) was explored in response to shear stress. Bovine aortic ECs(BAECs) were co-transfected with plasmids that encode a luciferease reporter gene controlled by c-fos promoter and the dominant-negative mutants of Rho, Cdc42, or Rac. Pre-treatment of BAECs with genistein, cytochalasin, or colchicine did not affect the shear induction of the c-fos promoter activity. It was found that shear-induced c-fos promoter activation is regulated by Rho and calcium, but is independent of Rac, Cdc42, cytoskeleton, and tyrosine kinase pathways.
|Original language||English (US)|
|Journal||Annals of Biomedical Engineering|
|Issue number||SUPPL. 1|
|State||Published - 2000|
ASJC Scopus subject areas
- Biomedical Engineering