TY - JOUR
T1 - Shear stress-induced c-fos promoter activation is regulated by Rho GTPases and intracellular calcium
AU - Shiu, Yang Ting Elizabeth
AU - Li, Song
AU - Wang, Yingxiao
AU - Yuan, Suli
AU - Chen, Benjamin
AU - Chien, Shu
PY - 2000
Y1 - 2000
N2 - In this study, the molecular basis of c-fos promoter activation in endothelial cells (ECs) was explored in response to shear stress. Bovine aortic ECs(BAECs) were co-transfected with plasmids that encode a luciferease reporter gene controlled by c-fos promoter and the dominant-negative mutants of Rho, Cdc42, or Rac. Pre-treatment of BAECs with genistein, cytochalasin, or colchicine did not affect the shear induction of the c-fos promoter activity. It was found that shear-induced c-fos promoter activation is regulated by Rho and calcium, but is independent of Rac, Cdc42, cytoskeleton, and tyrosine kinase pathways.
AB - In this study, the molecular basis of c-fos promoter activation in endothelial cells (ECs) was explored in response to shear stress. Bovine aortic ECs(BAECs) were co-transfected with plasmids that encode a luciferease reporter gene controlled by c-fos promoter and the dominant-negative mutants of Rho, Cdc42, or Rac. Pre-treatment of BAECs with genistein, cytochalasin, or colchicine did not affect the shear induction of the c-fos promoter activity. It was found that shear-induced c-fos promoter activation is regulated by Rho and calcium, but is independent of Rac, Cdc42, cytoskeleton, and tyrosine kinase pathways.
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M3 - Conference article
AN - SCOPUS:0034506908
SN - 0090-6964
VL - 28
SP - S-75
JO - Annals of biomedical engineering
JF - Annals of biomedical engineering
IS - SUPPL. 1
T2 - 2000 Annual Fall Meeting of the Biomedical Engineering Society
Y2 - 12 October 2000 through 14 October 2000
ER -