Short hairpin RNA targeting c-FLIP sensitizes human cervical adenocarcinoma Hela cells to chemotherapy and radiotherapy

Aiyue Luo, Wei Wang, Ni Sima, Yunping Lu, Jianfeng Zhou, Gang Xu, Hongtao Yu, Shixuan Wang, Ding Ma

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

c-FLIP inhibits caspase-8 activation and cell apoptosis mediated by death receptors. The present study aims at determining the effects of c-FLIP targeted vector-based short hairpin RNA (shRNA) on cell growth and evaluating its modulation of responsiveness to drugs and radiotherapy in cervical adenocarcinoma Hela cells. cFLIP expression of the cells transfected with shRNA against c-FLIP was significantly down-regulated after 72 h. c-FLIP silencing markedly suppressed cell proliferation and increased cell apoptosis. The activation of caspase-8 and caspase-3 was induced with shRNA targeting cFLIP with the passage of time after transfection. Furthermore, Vector-based shRNA against c-FLIP subsequently increased the sensitivity to cisplatin, iritican and Co60 radiotherapy by about 4- to 6-folds in Hela cells. Our data suggest that vector-based shRNA effectively inhibited c-FLIP expression, enhanced the expression level of caspase-8 and caspase-3 to induce cell apoptosis, probably with the higher efficacy in combination therapies with conventional chemotherapy and radiotherapy in cervical adenocarcinoma.

Original languageEnglish (US)
Pages (from-to)323-332
Number of pages10
JournalCancer Letters
Volume271
Issue number2
DOIs
StatePublished - Nov 28 2008

Keywords

  • Apoptosis
  • Cervical adenocarcinoma
  • cFLIP
  • shRNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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