Short Soluble Coumarin Crosslinkers for Light-Controlled Release of Cells and Proteins from Hydrogels

Caroline De Gracia Lux, Jacques Lux, Guillaume Collet, Sha He, Minnie Chan, Jason Olejniczak, Alexandra Foucault-Collet, Adah Almutairi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Materials that degrade or dissociate in response to low power light promise to enable on-demand, precisely localized delivery of drugs or bioactive molecules in living systems. Such applications remain elusive because few materials respond to wavelengths that appreciably penetrate tissues. The photocage bromohydroxycoumarin (Bhc) is efficiently cleaved upon low-power ultraviolet (UV) and near-infrared (NIR) irradiation through one- or two-photon excitation, respectively. We have designed and synthesized a short Bhc-bearing crosslinker to create light-degradable hydrogels and nanogels. Our crosslinker breaks by intramolecular cyclization in a manner inspired by the naturally occurring ornithine lactamization, in response to UV and NIR light, enabling rapid degradation of polyacrylamide gels and release of small hydrophilic payloads such as an 10 nm model protein and murine mesenchymal stem cells, with no background leakage.

Original languageEnglish (US)
Pages (from-to)3286-3296
Number of pages11
JournalBiomacromolecules
Volume16
Issue number10
DOIs
StatePublished - Oct 12 2015

Fingerprint

Hydrogels
Proteins
Bearings (structural)
Photons
Infrared radiation
Ornithine
Cyclization
Polyacrylates
Stem cells
Gels
Irradiation
Tissue
Degradation
Wavelength
Molecules
Pharmaceutical Preparations
coumarin

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Short Soluble Coumarin Crosslinkers for Light-Controlled Release of Cells and Proteins from Hydrogels. / De Gracia Lux, Caroline; Lux, Jacques; Collet, Guillaume; He, Sha; Chan, Minnie; Olejniczak, Jason; Foucault-Collet, Alexandra; Almutairi, Adah.

In: Biomacromolecules, Vol. 16, No. 10, 12.10.2015, p. 3286-3296.

Research output: Contribution to journalArticle

De Gracia Lux, Caroline ; Lux, Jacques ; Collet, Guillaume ; He, Sha ; Chan, Minnie ; Olejniczak, Jason ; Foucault-Collet, Alexandra ; Almutairi, Adah. / Short Soluble Coumarin Crosslinkers for Light-Controlled Release of Cells and Proteins from Hydrogels. In: Biomacromolecules. 2015 ; Vol. 16, No. 10. pp. 3286-3296.
@article{bd8b6a3e3b5d48f69386d0a14b66c15f,
title = "Short Soluble Coumarin Crosslinkers for Light-Controlled Release of Cells and Proteins from Hydrogels",
abstract = "Materials that degrade or dissociate in response to low power light promise to enable on-demand, precisely localized delivery of drugs or bioactive molecules in living systems. Such applications remain elusive because few materials respond to wavelengths that appreciably penetrate tissues. The photocage bromohydroxycoumarin (Bhc) is efficiently cleaved upon low-power ultraviolet (UV) and near-infrared (NIR) irradiation through one- or two-photon excitation, respectively. We have designed and synthesized a short Bhc-bearing crosslinker to create light-degradable hydrogels and nanogels. Our crosslinker breaks by intramolecular cyclization in a manner inspired by the naturally occurring ornithine lactamization, in response to UV and NIR light, enabling rapid degradation of polyacrylamide gels and release of small hydrophilic payloads such as an 10 nm model protein and murine mesenchymal stem cells, with no background leakage.",
author = "{De Gracia Lux}, Caroline and Jacques Lux and Guillaume Collet and Sha He and Minnie Chan and Jason Olejniczak and Alexandra Foucault-Collet and Adah Almutairi",
year = "2015",
month = "10",
day = "12",
doi = "10.1021/acs.biomac.5b00950",
language = "English (US)",
volume = "16",
pages = "3286--3296",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "10",

}

TY - JOUR

T1 - Short Soluble Coumarin Crosslinkers for Light-Controlled Release of Cells and Proteins from Hydrogels

AU - De Gracia Lux, Caroline

AU - Lux, Jacques

AU - Collet, Guillaume

AU - He, Sha

AU - Chan, Minnie

AU - Olejniczak, Jason

AU - Foucault-Collet, Alexandra

AU - Almutairi, Adah

PY - 2015/10/12

Y1 - 2015/10/12

N2 - Materials that degrade or dissociate in response to low power light promise to enable on-demand, precisely localized delivery of drugs or bioactive molecules in living systems. Such applications remain elusive because few materials respond to wavelengths that appreciably penetrate tissues. The photocage bromohydroxycoumarin (Bhc) is efficiently cleaved upon low-power ultraviolet (UV) and near-infrared (NIR) irradiation through one- or two-photon excitation, respectively. We have designed and synthesized a short Bhc-bearing crosslinker to create light-degradable hydrogels and nanogels. Our crosslinker breaks by intramolecular cyclization in a manner inspired by the naturally occurring ornithine lactamization, in response to UV and NIR light, enabling rapid degradation of polyacrylamide gels and release of small hydrophilic payloads such as an 10 nm model protein and murine mesenchymal stem cells, with no background leakage.

AB - Materials that degrade or dissociate in response to low power light promise to enable on-demand, precisely localized delivery of drugs or bioactive molecules in living systems. Such applications remain elusive because few materials respond to wavelengths that appreciably penetrate tissues. The photocage bromohydroxycoumarin (Bhc) is efficiently cleaved upon low-power ultraviolet (UV) and near-infrared (NIR) irradiation through one- or two-photon excitation, respectively. We have designed and synthesized a short Bhc-bearing crosslinker to create light-degradable hydrogels and nanogels. Our crosslinker breaks by intramolecular cyclization in a manner inspired by the naturally occurring ornithine lactamization, in response to UV and NIR light, enabling rapid degradation of polyacrylamide gels and release of small hydrophilic payloads such as an 10 nm model protein and murine mesenchymal stem cells, with no background leakage.

UR - http://www.scopus.com/inward/record.url?scp=84943744674&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943744674&partnerID=8YFLogxK

U2 - 10.1021/acs.biomac.5b00950

DO - 10.1021/acs.biomac.5b00950

M3 - Article

C2 - 26349005

AN - SCOPUS:84943744674

VL - 16

SP - 3286

EP - 3296

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 10

ER -