Short Soluble Coumarin Crosslinkers for Light-Controlled Release of Cells and Proteins from Hydrogels

Caroline De Gracia Lux, Jacques Lux, Guillaume Collet, Sha He, Minnie Chan, Jason Olejniczak, Alexandra Foucault-Collet, Adah Almutairi

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Materials that degrade or dissociate in response to low power light promise to enable on-demand, precisely localized delivery of drugs or bioactive molecules in living systems. Such applications remain elusive because few materials respond to wavelengths that appreciably penetrate tissues. The photocage bromohydroxycoumarin (Bhc) is efficiently cleaved upon low-power ultraviolet (UV) and near-infrared (NIR) irradiation through one- or two-photon excitation, respectively. We have designed and synthesized a short Bhc-bearing crosslinker to create light-degradable hydrogels and nanogels. Our crosslinker breaks by intramolecular cyclization in a manner inspired by the naturally occurring ornithine lactamization, in response to UV and NIR light, enabling rapid degradation of polyacrylamide gels and release of small hydrophilic payloads such as an 10 nm model protein and murine mesenchymal stem cells, with no background leakage.

Original languageEnglish (US)
Pages (from-to)3286-3296
Number of pages11
JournalBiomacromolecules
Volume16
Issue number10
DOIs
StatePublished - Oct 12 2015

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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