TY - JOUR
T1 - Short Soluble Coumarin Crosslinkers for Light-Controlled Release of Cells and Proteins from Hydrogels
AU - De Gracia Lux, Caroline
AU - Lux, Jacques
AU - Collet, Guillaume
AU - He, Sha
AU - Chan, Minnie
AU - Olejniczak, Jason
AU - Foucault-Collet, Alexandra
AU - Almutairi, Adah
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/10/12
Y1 - 2015/10/12
N2 - Materials that degrade or dissociate in response to low power light promise to enable on-demand, precisely localized delivery of drugs or bioactive molecules in living systems. Such applications remain elusive because few materials respond to wavelengths that appreciably penetrate tissues. The photocage bromohydroxycoumarin (Bhc) is efficiently cleaved upon low-power ultraviolet (UV) and near-infrared (NIR) irradiation through one- or two-photon excitation, respectively. We have designed and synthesized a short Bhc-bearing crosslinker to create light-degradable hydrogels and nanogels. Our crosslinker breaks by intramolecular cyclization in a manner inspired by the naturally occurring ornithine lactamization, in response to UV and NIR light, enabling rapid degradation of polyacrylamide gels and release of small hydrophilic payloads such as an 10 nm model protein and murine mesenchymal stem cells, with no background leakage.
AB - Materials that degrade or dissociate in response to low power light promise to enable on-demand, precisely localized delivery of drugs or bioactive molecules in living systems. Such applications remain elusive because few materials respond to wavelengths that appreciably penetrate tissues. The photocage bromohydroxycoumarin (Bhc) is efficiently cleaved upon low-power ultraviolet (UV) and near-infrared (NIR) irradiation through one- or two-photon excitation, respectively. We have designed and synthesized a short Bhc-bearing crosslinker to create light-degradable hydrogels and nanogels. Our crosslinker breaks by intramolecular cyclization in a manner inspired by the naturally occurring ornithine lactamization, in response to UV and NIR light, enabling rapid degradation of polyacrylamide gels and release of small hydrophilic payloads such as an 10 nm model protein and murine mesenchymal stem cells, with no background leakage.
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U2 - 10.1021/acs.biomac.5b00950
DO - 10.1021/acs.biomac.5b00950
M3 - Article
C2 - 26349005
AN - SCOPUS:84943744674
SN - 1525-7797
VL - 16
SP - 3286
EP - 3296
JO - Biomacromolecules
JF - Biomacromolecules
IS - 10
ER -