Signal transduction from small particles: Sulfur nanodots featuring mercury sensing, cell entry mechanism and in vitro tracking performance

Gongxi Qiao, Lei Liu, Xiaoxing Hao, Junke Zheng, Wanqiang Liu, Jinwei Gao, Cheng Cheng Zhang, Qianming Wang

Research output: Contribution to journalArticle

Abstract

As the standard for heavy metal sensing systems increases, it becomes more important to find out new self-indicating materials as alternatives. The traditional design of optical probe contains receptor and fluorophore as two moieties. Here a novel sulfur quantum dot as the intrinsic fluorescent sensor has been established and the signal transduction involves interaction of mercury ions with sulfur atoms which belongs to the internal system of the fluorophore. Its analytical performances can be assessed by fluorometric and colorimetric titrations simultaneously and the detection limits for Hg2+ ions are determined to be 65 nM and 1.86 μM respectively. In the presence of sulfur dots, very low cytotoxicity has been verified in two cell lines (HeLa and K562) via both cell counting kit-8 method and flow cytometry. For the first time, four sorts of chemical inhibitors have been employed to clarify the endocytosis mechanism based on sulfur quantum dots. The collected results indicate that clathrin and lipid raft mediated endocytosis play as primary roles in the internalization processes. It has been found that sulfur dots are located in the cytoplasm with intense blue emissions and the cellular tracking of mercury ions has been realized.

Original languageEnglish (US)
Article number122907
JournalChemical Engineering Journal
Volume382
DOIs
StatePublished - Feb 15 2020

Fingerprint

Signal transduction
Mercury
Sulfur
sulfur
Fluorophores
Ions
Semiconductor quantum dots
ion
Clathrin
Flow cytometry
flow cytometry
cytoplasm
Cytotoxicity
Heavy Metals
Titration
Lipids
Heavy metals
inhibitor
lipid
Cells

Keywords

  • Fluorescence
  • Mercury ions
  • Quantum dots
  • Sensor
  • Sulfur

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

Signal transduction from small particles : Sulfur nanodots featuring mercury sensing, cell entry mechanism and in vitro tracking performance. / Qiao, Gongxi; Liu, Lei; Hao, Xiaoxing; Zheng, Junke; Liu, Wanqiang; Gao, Jinwei; Zhang, Cheng Cheng; Wang, Qianming.

In: Chemical Engineering Journal, Vol. 382, 122907, 15.02.2020.

Research output: Contribution to journalArticle

Qiao, Gongxi ; Liu, Lei ; Hao, Xiaoxing ; Zheng, Junke ; Liu, Wanqiang ; Gao, Jinwei ; Zhang, Cheng Cheng ; Wang, Qianming. / Signal transduction from small particles : Sulfur nanodots featuring mercury sensing, cell entry mechanism and in vitro tracking performance. In: Chemical Engineering Journal. 2020 ; Vol. 382.
@article{b7bdb2f4f9b14484aacd0fc6cc8a1a5f,
title = "Signal transduction from small particles: Sulfur nanodots featuring mercury sensing, cell entry mechanism and in vitro tracking performance",
abstract = "As the standard for heavy metal sensing systems increases, it becomes more important to find out new self-indicating materials as alternatives. The traditional design of optical probe contains receptor and fluorophore as two moieties. Here a novel sulfur quantum dot as the intrinsic fluorescent sensor has been established and the signal transduction involves interaction of mercury ions with sulfur atoms which belongs to the internal system of the fluorophore. Its analytical performances can be assessed by fluorometric and colorimetric titrations simultaneously and the detection limits for Hg2+ ions are determined to be 65 nM and 1.86 μM respectively. In the presence of sulfur dots, very low cytotoxicity has been verified in two cell lines (HeLa and K562) via both cell counting kit-8 method and flow cytometry. For the first time, four sorts of chemical inhibitors have been employed to clarify the endocytosis mechanism based on sulfur quantum dots. The collected results indicate that clathrin and lipid raft mediated endocytosis play as primary roles in the internalization processes. It has been found that sulfur dots are located in the cytoplasm with intense blue emissions and the cellular tracking of mercury ions has been realized.",
keywords = "Fluorescence, Mercury ions, Quantum dots, Sensor, Sulfur",
author = "Gongxi Qiao and Lei Liu and Xiaoxing Hao and Junke Zheng and Wanqiang Liu and Jinwei Gao and Zhang, {Cheng Cheng} and Qianming Wang",
year = "2020",
month = "2",
day = "15",
doi = "10.1016/j.cej.2019.122907",
language = "English (US)",
volume = "382",
journal = "Chemical Engineering Journal",
issn = "1385-8947",
publisher = "Elsevier",

}

TY - JOUR

T1 - Signal transduction from small particles

T2 - Sulfur nanodots featuring mercury sensing, cell entry mechanism and in vitro tracking performance

AU - Qiao, Gongxi

AU - Liu, Lei

AU - Hao, Xiaoxing

AU - Zheng, Junke

AU - Liu, Wanqiang

AU - Gao, Jinwei

AU - Zhang, Cheng Cheng

AU - Wang, Qianming

PY - 2020/2/15

Y1 - 2020/2/15

N2 - As the standard for heavy metal sensing systems increases, it becomes more important to find out new self-indicating materials as alternatives. The traditional design of optical probe contains receptor and fluorophore as two moieties. Here a novel sulfur quantum dot as the intrinsic fluorescent sensor has been established and the signal transduction involves interaction of mercury ions with sulfur atoms which belongs to the internal system of the fluorophore. Its analytical performances can be assessed by fluorometric and colorimetric titrations simultaneously and the detection limits for Hg2+ ions are determined to be 65 nM and 1.86 μM respectively. In the presence of sulfur dots, very low cytotoxicity has been verified in two cell lines (HeLa and K562) via both cell counting kit-8 method and flow cytometry. For the first time, four sorts of chemical inhibitors have been employed to clarify the endocytosis mechanism based on sulfur quantum dots. The collected results indicate that clathrin and lipid raft mediated endocytosis play as primary roles in the internalization processes. It has been found that sulfur dots are located in the cytoplasm with intense blue emissions and the cellular tracking of mercury ions has been realized.

AB - As the standard for heavy metal sensing systems increases, it becomes more important to find out new self-indicating materials as alternatives. The traditional design of optical probe contains receptor and fluorophore as two moieties. Here a novel sulfur quantum dot as the intrinsic fluorescent sensor has been established and the signal transduction involves interaction of mercury ions with sulfur atoms which belongs to the internal system of the fluorophore. Its analytical performances can be assessed by fluorometric and colorimetric titrations simultaneously and the detection limits for Hg2+ ions are determined to be 65 nM and 1.86 μM respectively. In the presence of sulfur dots, very low cytotoxicity has been verified in two cell lines (HeLa and K562) via both cell counting kit-8 method and flow cytometry. For the first time, four sorts of chemical inhibitors have been employed to clarify the endocytosis mechanism based on sulfur quantum dots. The collected results indicate that clathrin and lipid raft mediated endocytosis play as primary roles in the internalization processes. It has been found that sulfur dots are located in the cytoplasm with intense blue emissions and the cellular tracking of mercury ions has been realized.

KW - Fluorescence

KW - Mercury ions

KW - Quantum dots

KW - Sensor

KW - Sulfur

UR - http://www.scopus.com/inward/record.url?scp=85072865099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072865099&partnerID=8YFLogxK

U2 - 10.1016/j.cej.2019.122907

DO - 10.1016/j.cej.2019.122907

M3 - Article

AN - SCOPUS:85072865099

VL - 382

JO - Chemical Engineering Journal

JF - Chemical Engineering Journal

SN - 1385-8947

M1 - 122907

ER -