TY - JOUR
T1 - Significantly improved resolution for noe correlations from valine and isoleucine (C(y)2) methyl groups in 15N, 13C- and 15N, 13C, 2H-labeled proteins
AU - Zwahlen, Catherine
AU - Vincent, Sébastien J F
AU - Gardner, Kevin H.
AU - Kay, Lewis E.
PY - 1998/5/20
Y1 - 1998/5/20
N2 - A new NMR experiment is described for recording NOEs from Val and Ile methyl groups in 15N, 13C-labeled or methyl-protonated, 15N, 13C, 2H-labeled proteins that offers far superior resolution than conventional 3D 13C-edited NOESY data sets. Resolution is achieved by recording both the C(β) and C(γ) (Val) or C(γ2) (Ile) chemical shifts as well as the chemical shift of the destination proton, and a strategy is introduced for refocusing homonuclear carbon couplings during the constant-time evolution of C(β) carbon magnetization. The utility of the method is demonstrated with applications on a 160- residue fully proton areal 15N, 13C-labeled, dNumb PTB domain- peptide complex and a methyl protonated, highly deuterated 15N, 13C-labeled complex of maltose binding protein and β-cyclodextrin (42 kDa).
AB - A new NMR experiment is described for recording NOEs from Val and Ile methyl groups in 15N, 13C-labeled or methyl-protonated, 15N, 13C, 2H-labeled proteins that offers far superior resolution than conventional 3D 13C-edited NOESY data sets. Resolution is achieved by recording both the C(β) and C(γ) (Val) or C(γ2) (Ile) chemical shifts as well as the chemical shift of the destination proton, and a strategy is introduced for refocusing homonuclear carbon couplings during the constant-time evolution of C(β) carbon magnetization. The utility of the method is demonstrated with applications on a 160- residue fully proton areal 15N, 13C-labeled, dNumb PTB domain- peptide complex and a methyl protonated, highly deuterated 15N, 13C-labeled complex of maltose binding protein and β-cyclodextrin (42 kDa).
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U2 - 10.1021/ja9742601
DO - 10.1021/ja9742601
M3 - Article
AN - SCOPUS:0032550614
SN - 0002-7863
VL - 120
SP - 4825
EP - 4831
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 19
ER -