Silence of chromosomal amplifications in colon cancer

Petra Platzer, Madhvi B. Upender, Keith Wilson, Joseph Willis, James Lutterbaugh, Arman Nosrati, James K V Willson, David Mack, Thomas Ried, Sanford Markowitz

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135 Scopus citations

Abstract

Oncogene activation by gene amplification is a major pathogenetic mechanism in human cancer. Using comparative genomic hybridization, we determined that metastatic human colon cancers commonly acquire numerous extra copies of chromosome arms 7p, 8q, 13q, and 20q. We then examined the consequence of these amplifications on gene expression using DNA microarrays. Of 55,000 transcripts profiled, 2,146 were determined to map to one of the four common colon cancer amplicons and to also be expressed in normal or malignant colon tissues. Of these, only 81 transcripts (3.8%) demonstrated a 2-fold increase over normal expression among cancers bearing the corresponding chromosomal amplification. Chromosomal amplifications are common in colon cancer metastasis, but increased expression of genes within these amplicons is rare.

Original languageEnglish (US)
Pages (from-to)1134-1138
Number of pages5
JournalCancer Research
Volume62
Issue number4
Publication statusPublished - 2002

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Platzer, P., Upender, M. B., Wilson, K., Willis, J., Lutterbaugh, J., Nosrati, A., ... Markowitz, S. (2002). Silence of chromosomal amplifications in colon cancer. Cancer Research, 62(4), 1134-1138.