Silencing gene expression by targeting chromosomal DNA with antigene peptide nucleic acids and duplex RNAs

Bethany A. Janowski, Jiaxin Hu, David R. Corey

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The value of recognizing cellular RNA sequences by short interfering RNAs (siRNAs) in mammalian cells is widely appreciated, but what might be learned if it were also possible to recognize chromosomal DNA? Recognition of chromosomal DNA would have many applications, such as inhibiting gene expression, activating gene expression, introducing mutations, and probing chromosome structure and function. We have shown that antigene peptide nucleic acids (agPNAs) and antigene duplex RNAs (agRNAs) block gene expression and probe chromosomal DNA. Here we describe a protocol for designing antigene agents and introducing them into cells. This protocol can also be used to silence expression with PNAs or siRNAs that target mRNA. From preparation of oligomers to analysis of data, experiments with agPNAs and agRNAs require ∼14 d and 9 d, respectively.

Original languageEnglish (US)
Pages (from-to)436-443
Number of pages8
JournalNature Protocols
Volume1
Issue number1
DOIs
StatePublished - Jun 2006

Fingerprint

Peptide Nucleic Acids
Gene Targeting
Gene expression
DNA Probes
RNA
Gene Expression
Small Interfering RNA
DNA
Chromosome Structures
Chromosomes
Oligomers
Cells
Messenger RNA
Mutation
Experiments

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Silencing gene expression by targeting chromosomal DNA with antigene peptide nucleic acids and duplex RNAs. / Janowski, Bethany A.; Hu, Jiaxin; Corey, David R.

In: Nature Protocols, Vol. 1, No. 1, 06.2006, p. 436-443.

Research output: Contribution to journalArticle

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