Silencing of filamin A gene expression inhibits Ca2+-sensing receptor signaling

Chunfa Huang, Zhenzhen Wu, Kristine M. Hujer, R. Tyler Miller

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca2+-sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function.

Original languageEnglish (US)
Pages (from-to)1795-1800
Number of pages6
JournalFEBS Letters
Volume580
Issue number7
DOIs
StatePublished - Mar 20 2006

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Keywords

  • Ca-sensing receptor
  • Filamin A
  • MAP kinase
  • Signaling
  • siRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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