Silencing of filamin A gene expression inhibits Ca2+-sensing receptor signaling

Chunfa Huang; Zhenzhen Wu; Kristine M. Hujer; R. Tyler Miller

doi:10.1016/j.febslet.2006.02.035

Silencing of filamin A gene expression inhibits Ca²⁺-sensing receptor signaling

Chunfa Huang, Zhenzhen Wu, Kristine M. Hujer, R. Tyler Miller

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca²⁺-sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function.

Original language	English (US)
Pages (from-to)	1795-1800
Number of pages	6
Journal	FEBS Letters
Volume	580
Issue number	7
DOIs	https://doi.org/10.1016/j.febslet.2006.02.035
State	Published - Mar 20 2006

Keywords

Ca-sensing receptor
Filamin A
MAP kinase
Signaling
siRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/j.febslet.2006.02.035

Cite this

@article{e815494b9fc64006b3365bf0aa3b128e,

title = "Silencing of filamin A gene expression inhibits Ca2+-sensing receptor signaling",

abstract = "Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca2+-sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function.",

keywords = "Ca-sensing receptor, Filamin A, MAP kinase, Signaling, siRNA",

author = "Chunfa Huang and Zhenzhen Wu and Hujer, {Kristine M.} and Miller, {R. Tyler}",

note = "Funding Information: This work was supported by grants from the National Institutes of Health (DK-59985 and HL-41618), a VA Merit Review Award to R.T. Miller, and the Leonard Rosenberg Research Foundation.",

year = "2006",

month = mar,

day = "20",

doi = "10.1016/j.febslet.2006.02.035",

language = "English (US)",

volume = "580",

pages = "1795--1800",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "7",

}

TY - JOUR

T1 - Silencing of filamin A gene expression inhibits Ca2+-sensing receptor signaling

AU - Huang, Chunfa

AU - Wu, Zhenzhen

AU - Hujer, Kristine M.

AU - Miller, R. Tyler

N1 - Funding Information: This work was supported by grants from the National Institutes of Health (DK-59985 and HL-41618), a VA Merit Review Award to R.T. Miller, and the Leonard Rosenberg Research Foundation.

PY - 2006/3/20

Y1 - 2006/3/20

N2 - Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca2+-sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function.

AB - Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca2+-sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function.

KW - Ca-sensing receptor

KW - Filamin A

KW - MAP kinase

KW - Signaling

KW - siRNA

UR - http://www.scopus.com/inward/record.url?scp=33644895118&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644895118&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2006.02.035

DO - 10.1016/j.febslet.2006.02.035

M3 - Article

C2 - 16513120

AN - SCOPUS:33644895118

SN - 0014-5793

VL - 580

SP - 1795

EP - 1800

JO - FEBS Letters

JF - FEBS Letters

IS - 7

ER -