Similarities of Drosophila rab GTPases based on expression profiling

Completion and analysis of the rab-Gal4 kit

Eugene Jennifer Jin, Chih Chiang Chan, Egemen Agi, Smita Cherry, Elizabeth Hanacik, Michael Buszczak, P. Robin Hiesinger

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We recently generated rab-Gal4 lines for 25 of 29 predicted Drosophila rab GTPases. These lines provide tools for the expression of reporters, mutant rab variants or other genes, under control of the regulatory elements of individual rab loci. Here, we report the generation and characterization of the remaining four rab-Gal4 lines. Based on the completed 'rab-Gal4 kit' we performed a comparative analysis of the cellular and subcellular expression of all rab GTPases. This analysis includes the cellular expression patterns in characterized neuronal and non-neuronal cells and tissues, the subcellular localization of wild type, constitutively active and dominant negative rab GTPases and colocalization with known intracellular compartment markers. Our comparative analysis identifies all Rab GTPases that are expressed in the same cells and localize to the same intracellular compartments. Remarkably, similarities based on these criteria are typically not predicted by primary sequence homology. Hence, our findings provide an alternative basis to assess potential roles and redundancies based on expression in developing and adult cell types, compartment identity and subcellular localization.

Original languageEnglish (US)
Article numbere40912
JournalPLoS One
Volume7
Issue number7
DOIs
StatePublished - Jul 23 2012

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rab GTP-Binding Proteins
guanosinetriphosphatase
Drosophila
cells
Sequence Homology
sequence homology
Redundancy
Genes
Tissue
mutants
loci
genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Similarities of Drosophila rab GTPases based on expression profiling : Completion and analysis of the rab-Gal4 kit. / Jin, Eugene Jennifer; Chan, Chih Chiang; Agi, Egemen; Cherry, Smita; Hanacik, Elizabeth; Buszczak, Michael; Hiesinger, P. Robin.

In: PLoS One, Vol. 7, No. 7, e40912, 23.07.2012.

Research output: Contribution to journalArticle

Jin, Eugene Jennifer ; Chan, Chih Chiang ; Agi, Egemen ; Cherry, Smita ; Hanacik, Elizabeth ; Buszczak, Michael ; Hiesinger, P. Robin. / Similarities of Drosophila rab GTPases based on expression profiling : Completion and analysis of the rab-Gal4 kit. In: PLoS One. 2012 ; Vol. 7, No. 7.
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