Simple purification of adeno-associated virus-DJ for liver-specific gene expression

Jingjing Liu, Young Ah Moon

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Recombinant gene expression using adeno-associated viruses (AAVs) has become a valuable tool in animal studies, as they mediate safe expression of transduced genes for several months. The liver is a major organ of metabolism, and liver-specific expression of a gene can be an invaluable tool for metabolic studies. AAV-DJ is a recombinant AAV generated by the gene shuffling of various AAV serotypes and shares characteristics of AAV2 and AAV8. AAV-DJ contains a heparin-binding domain in its capsid, which suggests that a heparin column could be used for the purification of the AAV. Given that AAV-DJ has been only recently available, relatively little is known about the optimal preparation/purification and application of AAV-DJ. Here, we present a simple large-scale preparation method that can generate 3×1013 viral particles for in vivo experiments and demonstrate liver-specific gene expression via systemic injection in mice.

Original languageEnglish (US)
Pages (from-to)790-794
Number of pages5
JournalYonsei Medical Journal
Volume57
Issue number3
DOIs
Publication statusPublished - May 1 2016

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Keywords

  • AAV-DJ
  • Large scale production
  • Liver-specific expression

ASJC Scopus subject areas

  • Medicine(all)

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