Simulating obstructive sleep apnea patients' oxygenation characteristics into a mouse model of cyclical intermittent hypoxia

Diane C. Lim, Daniel C. Brady, Pengse Po, Li Pang Chuang, Laise Marcondes, Emily Y. Kim, Brendan T. Keenan, Xiaofeng Guo, Greg Maislin, Raymond J. Galante, Allan I. Pack

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Mouse models of cyclical intermittent hypoxia (CIH) are used to study the consequences of both hypoxia and oxidative stress in obstructive sleep apnea (OSA). Whether or not a mouse model of CIH that simulates OSA patients' oxygenation characteristics would translate into improved patient care remains unanswered. First we identified oxygenation characteristics using the desaturation and resaturation time in 47 OSA subjects from the Molecular Signatures of Obstructive Sleep Apnea Cohort (MSOSA). We observe that a cycle of intermittent hypoxia is not sinusoidal; specifically, desaturation time increases in an almost linear relationship to the degree of hypoxia (nadir), whereas resaturation time is somewhat constant (∼15 s), irrespective of the nadir. Second, we modified the Hycon mouse model of CIH to accommodate a 15-s resaturation time. Using this modified CIH model, we explored whether a short resaturation schedule (15 s), which includes the characteristics of OSA patients, had a different effect on levels of oxidative stress (i.e., urinary 8,12-iso-iPF-VI levels) compared with sham and a long resaturation schedule (90 s), a schedule that is not uncommon in rodent models of CIH. Results suggest that shorter resaturation time may result in a higher level of 8,12-iso-iPF-VI compared with long resaturation or sham conditions. Therefore, simulating the rodent model of CIH to reflect this and other OSA patients' oxygenation characteristics may be worthy of consideration to better understand the effects of hypoxia, oxidative stress, and their interactions.

Original languageEnglish (US)
Pages (from-to)544-557
Number of pages14
JournalJournal of Applied Physiology
Volume118
Issue number5
DOIs
StatePublished - Mar 1 2015

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Obstructive Sleep Apnea
Appointments and Schedules
Oxidative Stress
Rodentia
Hydrocodone
Hypoxia
Patient Care

Keywords

  • 8,12-iso-iPF-VI
  • Cyclical intermittent hypoxia
  • Obstructive sleep apnea
  • Oxidative stress

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Simulating obstructive sleep apnea patients' oxygenation characteristics into a mouse model of cyclical intermittent hypoxia. / Lim, Diane C.; Brady, Daniel C.; Po, Pengse; Chuang, Li Pang; Marcondes, Laise; Kim, Emily Y.; Keenan, Brendan T.; Guo, Xiaofeng; Maislin, Greg; Galante, Raymond J.; Pack, Allan I.

In: Journal of Applied Physiology, Vol. 118, No. 5, 01.03.2015, p. 544-557.

Research output: Contribution to journalArticle

Lim, DC, Brady, DC, Po, P, Chuang, LP, Marcondes, L, Kim, EY, Keenan, BT, Guo, X, Maislin, G, Galante, RJ & Pack, AI 2015, 'Simulating obstructive sleep apnea patients' oxygenation characteristics into a mouse model of cyclical intermittent hypoxia', Journal of Applied Physiology, vol. 118, no. 5, pp. 544-557. https://doi.org/10.1152/japplphysiol.00629.2014
Lim, Diane C. ; Brady, Daniel C. ; Po, Pengse ; Chuang, Li Pang ; Marcondes, Laise ; Kim, Emily Y. ; Keenan, Brendan T. ; Guo, Xiaofeng ; Maislin, Greg ; Galante, Raymond J. ; Pack, Allan I. / Simulating obstructive sleep apnea patients' oxygenation characteristics into a mouse model of cyclical intermittent hypoxia. In: Journal of Applied Physiology. 2015 ; Vol. 118, No. 5. pp. 544-557.
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