Abstract
A novel liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-Q-TOFMS) method was developed for the simultaneous determination of 23 amino acids and 7 biogenic amines in food samples. These analytes were pre-column derivatized with dansyl chloride and then separated in an Acquity™ column (1.7μm; 2.1mm×100mm). The separation of 31 compounds including an internal standard was achieved within 25min at a flow rate of 0.2mL/min. The method linearity for each amino acid and biogenic amine had a relatively wide range with r 2>0.99. The intra- and inter-day precision, expressed as relative standard deviation (RSD), ranged from 1.1 to 4.6% and from 2.0 to 11.2%, respectively. The limit of detection was between 0.005 and 0.4μg/mL. With a simple dilution, recoveries of around 80-120% were obtained for most of the compounds. No significant matrix effect was observed, and the developed method was successfully applied to the analysis of amino acids and biogenic amines in beer, cheese and sausage samples.
Original language | English (US) |
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Pages (from-to) | 9174-9182 |
Number of pages | 9 |
Journal | Journal of Chromatography A |
Volume | 1218 |
Issue number | 51 |
DOIs | |
State | Published - Dec 23 2011 |
Keywords
- Amino acid
- Biogenic amine
- Dansyl chloride
- Q-TOFMS
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry