Simultaneous determination of 23 amino acids and 7 biogenic amines in fermented food samples by liquid chromatography/quadrupole time-of-flight mass spectrometry

Shaodong Jia, Yun Pyo Kang, Jeong Hill Park, Jeongmi Lee, Sung Won Kwon

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

A novel liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-Q-TOFMS) method was developed for the simultaneous determination of 23 amino acids and 7 biogenic amines in food samples. These analytes were pre-column derivatized with dansyl chloride and then separated in an Acquity™ column (1.7μm; 2.1mm×100mm). The separation of 31 compounds including an internal standard was achieved within 25min at a flow rate of 0.2mL/min. The method linearity for each amino acid and biogenic amine had a relatively wide range with r 2>0.99. The intra- and inter-day precision, expressed as relative standard deviation (RSD), ranged from 1.1 to 4.6% and from 2.0 to 11.2%, respectively. The limit of detection was between 0.005 and 0.4μg/mL. With a simple dilution, recoveries of around 80-120% were obtained for most of the compounds. No significant matrix effect was observed, and the developed method was successfully applied to the analysis of amino acids and biogenic amines in beer, cheese and sausage samples.

Original languageEnglish (US)
Pages (from-to)9174-9182
Number of pages9
JournalJournal of Chromatography A
Volume1218
Issue number51
DOIs
StatePublished - Dec 23 2011

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Keywords

  • Amino acid
  • Biogenic amine
  • Dansyl chloride
  • Q-TOFMS

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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