Simultaneous intracellular and extracellular pH measurement in the heart by 19F NMR of 6-fluoropyridoxol

Sandeep Hunjan, Ralph P. Mason, Vimal D. Mehta, Padmakar V. Kulkarni, Subramanian Aravind, Veeva Arora, Peter P. Antich

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

6-Fluoropyridoxol (6-FPOL) was evaluated as a simultaneous indicator of intracellular and extracellular pH and, hence, pH gradient in perfused rat hearts. After infusion, 19F NMR spectra rapidly showed two well-resolved peaks assigned to the intracellular and extracellular compartments, and pH was calculated on the basis of chemical shift with respect to a sodium trifluoroacetate standard. To demonstrate use of this molecule, dynamic changes in myocardial pH were assessed with a time resolution of 2 min during respiratory and metabolic alkalosis or acidosis and ischemia. For a typical heart, intracellular pH (pHi) = 7.14 ± 0.01 and extracellular pH (pHe) = 7.52 ± 0.02. In response to metabolic alkalosis, pHi remained relatively constant and the pH gradient increased. In contrast, respiratory challenge caused a significant increase in pHi. Independent measurements using pH electrodes and 31p NMR confirmed validity of the 19F NMR results.

Original languageEnglish (US)
Pages (from-to)551-556
Number of pages6
JournalMagnetic resonance in medicine
Volume39
Issue number4
DOIs
StatePublished - Apr 1998

Keywords

  • Fluorine
  • Nuclear magnetic resonance spectroscopy
  • pH gradient
  • pH indicator

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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