Simultaneous Profiling of Lysoglycerophospholipids in Rice (Oryza sativa L.) Using Direct Infusion-Tandem Mass Spectrometry with Multiple Reaction Monitoring

Dong Kyu Lim, Changyeun Mo, Nguyen Phuoc Long, Giyoung Kim, Sung Won Kwon

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

White rice is the final product after the hull and bran layers have been removed during the milling process. Although lysoglycerophospholipids (lysoGPLs) only occupy a small proportion in white rice, they are essential for evaluating rice authenticity and quality. In this study, we developed a high-throughput and targeted lipidomics approach that involved direct infusion-tandem mass spectrometry with multiple reaction monitoring to simultaneously profile lysoGPLs in white rice. The method is capable of characterizing 17 lysoGPLs within 1 min. In addition, unsupervised and supervised analyses exhibited a considerably large diversity of lysoGPL concentrations in white rice from different origins. In particular, a classification model was built using identified lysoGPLs that can differentiate white rice from Korea, China, and Japan. Among the discriminatory lysoGPLs, for the lysoPE(16:0) and lysoPE(18:2) compositions, there were relatively small within-group variations, and they were considerably different among the three countries. In conclusion, our proposed method provides a rapid, high-throughput, and comprehensive format for profiling lysoGPLs in rice samples.

Original languageEnglish (US)
Pages (from-to)2628-2634
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume65
Issue number12
DOIs
StatePublished - Mar 29 2017

Keywords

  • direct infusion-mass spectrometry
  • discriminatory marker
  • lysoglycerophospholipid
  • Oryza sativa L.
  • partial least-squares discriminant analysis

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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