TY - JOUR
T1 - Simultaneous profiling of lysoglycerophospholipids in rice (Oryza sativa L) using direct infusion-tandem mass spectrometry with multiple reaction monitoring
AU - Lim, Dong Kyu
AU - Mo, Changyeun
AU - Long, Nguyen Phuoc
AU - Kim, Giyoung
AU - Kwon, Sung Won
N1 - Funding Information:
*E-mail: swkwon@snu.ac.kr. ORCID Sung Won Kwon: 0000-0001-7161-4737 Funding This work was supported by the Rural Development Administration of Korea (PJ01164601), the National Research Foundation (NRF-2016R1E1A2020743), and the BK21 Plus Program in 2016. The authors confirm that the funder had no influence over the study design, content of the article, or selection of this journal. Notes The authors declare no competing financial interest.
Funding Information:
This work was supported by the Rural Development Administration of Korea (PJ01164601), the National Research Foundation (NRF-2016R1E1A2020743), and the BK21 Plus Program in 2016. The authors confirm that the funder had no influence over the study design, content of the article, or selection of this journal.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/3/29
Y1 - 2017/3/29
N2 - White rice is the final product after the hull and bran layers have been removed during the milling process. Although lysoglycerophospholipids (lysoGPLs) only occupy a small proportion in white rice, they are essential for evaluating rice authenticity and quality. In this study, we developed a high-throughput and targeted lipidomics approach that involved direct infusion-tandem mass spectrometry with multiple reaction monitoring to simultaneously profile lysoGPLs in white rice. The method is capable of characterizing 17 lysoGPLs within 1 min. In addition, unsupervised and supervised analyses exhibited a considerably large diversity of lysoGPL concentrations in white rice from different origins. In particular, a classification model was built using identified lysoGPLs that can differentiate white rice from Korea, China, and Japan. Among the discriminatory lysoGPLs, for the lysoPE(16:0) and lysoPE(18:2) compositions, there were relatively small within-group variations, and they were considerably different among the three countries. In conclusion, our proposed method provides a rapid, high-throughput, and comprehensive format for profiling lysoGPLs in rice samples.
AB - White rice is the final product after the hull and bran layers have been removed during the milling process. Although lysoglycerophospholipids (lysoGPLs) only occupy a small proportion in white rice, they are essential for evaluating rice authenticity and quality. In this study, we developed a high-throughput and targeted lipidomics approach that involved direct infusion-tandem mass spectrometry with multiple reaction monitoring to simultaneously profile lysoGPLs in white rice. The method is capable of characterizing 17 lysoGPLs within 1 min. In addition, unsupervised and supervised analyses exhibited a considerably large diversity of lysoGPL concentrations in white rice from different origins. In particular, a classification model was built using identified lysoGPLs that can differentiate white rice from Korea, China, and Japan. Among the discriminatory lysoGPLs, for the lysoPE(16:0) and lysoPE(18:2) compositions, there were relatively small within-group variations, and they were considerably different among the three countries. In conclusion, our proposed method provides a rapid, high-throughput, and comprehensive format for profiling lysoGPLs in rice samples.
KW - Direct infusion-mass spectrometry
KW - Discriminatory marker
KW - Lysoglycerophospholipid
KW - Oryza sativa L.
KW - Partial least-squares discriminant analysis
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U2 - 10.1021/acs.jafc.7b00148
DO - 10.1021/acs.jafc.7b00148
M3 - Article
C2 - 28245645
AN - SCOPUS:85021859503
SN - 0021-8561
VL - 65
SP - 2628
EP - 2634
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 12
ER -