Simultaneous profiling of lysoglycerophospholipids in rice (Oryza sativa L) using direct infusion-tandem mass spectrometry with multiple reaction monitoring

Dong Kyu Lim, Changyeun Mo, Nguyen Phuoc Long, Giyoung Kim, Sung Won Kwon

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

White rice is the final product after the hull and bran layers have been removed during the milling process. Although lysoglycerophospholipids (lysoGPLs) only occupy a small proportion in white rice, they are essential for evaluating rice authenticity and quality. In this study, we developed a high-throughput and targeted lipidomics approach that involved direct infusion-tandem mass spectrometry with multiple reaction monitoring to simultaneously profile lysoGPLs in white rice. The method is capable of characterizing 17 lysoGPLs within 1 min. In addition, unsupervised and supervised analyses exhibited a considerably large diversity of lysoGPL concentrations in white rice from different origins. In particular, a classification model was built using identified lysoGPLs that can differentiate white rice from Korea, China, and Japan. Among the discriminatory lysoGPLs, for the lysoPE(16:0) and lysoPE(18:2) compositions, there were relatively small within-group variations, and they were considerably different among the three countries. In conclusion, our proposed method provides a rapid, high-throughput, and comprehensive format for profiling lysoGPLs in rice samples.

Original languageEnglish (US)
Pages (from-to)2628-2634
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume65
Issue number12
DOIs
StatePublished - Mar 29 2017

Keywords

  • Direct infusion-mass spectrometry
  • Discriminatory marker
  • Lysoglycerophospholipid
  • Oryza sativa L.
  • Partial least-squares discriminant analysis

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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