Simultaneous visualization of Helicobacter pylori and gastric morphology: A new stain

Robert M. Genta, George O. Robason, David Y. Graham

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

Although the histopathologic patterns associated with Helicobacter pylori infection have been described, details of the interaction between bacteria, epithelial cells, and inflammatory cells remain elusive. One of the limiting factors has been the lack of a staining technique that allows the simultaneous visualization of H pylori and tissue morphology. By combining three commonly available stains (Steiner, hematoxylin-eosin, and alcian blue at pH 2.5) into a single procedure we have developed a stain that permits the optimal detection of H pylori in tissue sections while simultaneously allowing the histopathologic evaluation of all salient characteristics of the gastric mucosa. This procedure is inexpensive, as easy to perform as any silver stain, and provides consistent results. This stain is as sensitive as the Warthin-Starry stain for the detection of H pylori and significantly more sensitive than hematoxylin-eosin alone (>99% ν 85% when compared with the hematoxylin-eosin stain in a series of 332 positive biopsy specimens; >99% ν 61% in 49 biopsy specimens with rare bacteria). This stain is particularly useful for the visualization of small numbers of bacteria, such as in the evaluation of posttreatment gastric biopsy specimens, in specimens with abundant mucus or debris, and in specimens from the corpus, where H pylori usually does not elicit strong inflammatory responses and, therefore, pathologists' index of suspicion tends to be low.

Original languageEnglish (US)
Pages (from-to)221-226
Number of pages6
JournalHuman Pathology
Volume25
Issue number3
DOIs
StatePublished - 1994

Fingerprint

Helicobacter pylori
Stomach
Coloring Agents
Pylorus
Hematoxylin
Eosine Yellowish-(YS)
Bacteria
Biopsy
Alcian Blue
Helicobacter Infections
Mucus
Gastric Mucosa
Silver
Epithelial Cells
Staining and Labeling

Keywords

  • gastritis
  • Helicobacter pylori
  • staining
  • stomach

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Simultaneous visualization of Helicobacter pylori and gastric morphology : A new stain. / Genta, Robert M.; Robason, George O.; Graham, David Y.

In: Human Pathology, Vol. 25, No. 3, 1994, p. 221-226.

Research output: Contribution to journalArticle

Genta, Robert M. ; Robason, George O. ; Graham, David Y. / Simultaneous visualization of Helicobacter pylori and gastric morphology : A new stain. In: Human Pathology. 1994 ; Vol. 25, No. 3. pp. 221-226.
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