Single Bout Short Duration Fluid Shear Stress Induces Osteogenic Differentiation of MC3T3-E1 Cells via Integrin β1 and BMP2 Signaling Cross-Talk

Zhihui Mai, Zhuli Peng, Sihan Wu, Jinglan Zhang, Lin Chen, Huangyou Liang, Ding Bai, Guangmei Yan, Hong Ai

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Fluid shear stress plays an important role in bone osteogenic differentiation. It is traditionally believed that pulsed and continuous stress load is more favorable for fracture recovery and bone homeostasis. However, according to our clinical practice, we notice that one single stress load is also sufficient to trigger osteogenic differentiation. In the present study, we subject osteoblast MC3T3-E1 cells to single bout short duration fluid shear stress by using a parallel plate flow system. The results show that 1 hour of fluid shear stress at 12 dyn/cm2 promotes terminal osteogenic differentiation, including rearrangement of F-actin stress fiber, up-regulation of osteogenic genes expression, elevation of alkaline phosphatase activity, secretion of type I collagen and osteoid nodule formation. Moreover, collaboration of BMP2 and integrin β1 pathways plays a significant role in such differentiation processes. Our findings provide further experimental evidence to support the notion that single bout short duration fluid shear stress can promote osteogenic differentiation.

Original languageEnglish (US)
Article numbere61600
JournalPloS one
Volume8
Issue number4
DOIs
StatePublished - Apr 11 2013
Externally publishedYes

ASJC Scopus subject areas

  • General

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