Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets

NIH Intramural Sequencing Center

Research output: Contribution to journalArticlepeer-review

Abstract

Myelofibrosis (MF) is characterized by increased numbers of morphologically abnormal megakaryocytes (Mks). Single-cell RNA sequencing of >120,000 hematopoietic stem and progenitor cells demonstrated Mk-biased hematopoiesis across clinical and molecular MF subgroups. Mk progenitors were heterogeneous, with distinct expression of inflammatory mediators. Aberrant surface G6B expression on MF stem and progenitors could allow selective immunotherapeutic targeting of the MF clone.

Original languageEnglish (US)
Pages (from-to)477-492.e8
JournalMolecular cell
Volume78
Issue number3
DOIs
StatePublished - May 7 2020
Externally publishedYes

Keywords

  • G6B
  • TARGET-seq
  • bone marrow
  • fibrosis
  • immunotherapy
  • megakaryopoiesis
  • myeloproliferative neoplasm
  • platelets
  • single-cell multi-omics

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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