Single-cell RNA-sequencing of peripheral blood mononuclear cells with ddSEQ

Shaheen Khan, Kelly A. Kaihara

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Peripheral blood mononuclear cells (PBMCs) are blood cells that are a critical part of the immune system used to fight off infection. However, due to the complexity of PBMCs, which contain multiple different cell types, studying the function of the individual cell types can be difficult, and often studies rely on bulk measurements. Here, we describe the analysis of PBMCs using single-cell RNA-sequencing in droplets. Data from these studies allow for the identification and quantification of the subpopulation of cells that make up the PBMC sample. In addition, differential gene expression between cell types and samples can be assessed.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages155-176
Number of pages22
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1979
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • CD14 monocytes
  • PBMC
  • RNA-sequencing
  • Single-cell
  • ddSEQ

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Khan, S., & Kaihara, K. A. (2019). Single-cell RNA-sequencing of peripheral blood mononuclear cells with ddSEQ. In Methods in Molecular Biology (pp. 155-176). (Methods in Molecular Biology; Vol. 1979). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9240-9_10