Single-cell transcriptomic analysis of the immune cell landscape in the aged mouse brain after ischemic stroke

Xuan Li, Jingjun Lyu, Ran Li, Vaibhav Jain, Yuntian Shen, Ángela del Águila, Ulrike Hoffmann, Huaxin Sheng, Wei Yang

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Ischemic stroke is a medical emergency that primarily affects the elderly. A complex immune response in the post-stroke brain constitutes a key component of stroke pathophysiology. This study aimed to determine how stroke affects immune cell populations in the aged brain based on molecular profiles of individual cells. Methods: Single-cell RNA sequencing and a new transient ischemic stroke mouse model with late reperfusion were used. Results: We generated, for the first time, a composite picture of immune cell populations in the stroke aged brain at single-cell resolution. We discovered at least 6 microglial subsets in the stroke aged brain, including a potentially stroke-specific subtype. Moreover, we identified major cell subpopulations formed by infiltrated myeloid cells after stroke, and revealed their unique molecular profiles. Conclusions: This study provided the first scRNA-seq data set for immune cells in the stroke aged brain, and offered novel insights into post-stroke immune cell heterogeneity.

Original languageEnglish (US)
Article number83
JournalJournal of Neuroinflammation
Volume19
Issue number1
DOIs
StatePublished - Dec 2022
Externally publishedYes

Keywords

  • Dendritic cell
  • Late reperfusion
  • Microglia
  • Monocyte
  • Neuroinflammation
  • Neutrophil
  • scRNA-seq
  • Transcriptome

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology
  • Neurology
  • Cellular and Molecular Neuroscience

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