Single-channel currents of NMDA type activated by l- and d-homocysteic acid in cerebellar granule cells in culture

G. Kilić, M. Sciancalepore, E. Cherubini

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The whole-cell and the outside-out configurations of the patch-clamp technique were used to study macroscopic and single-channel currents evoked by the enantiomers of homocysteic acid, the sulphur containing analogue of glutamate, in cerebellar granule cells in culture. l-Homocysteic acid (l-HC, 15 μM) and d-homocysteic acid (d-HC, 50 μM) induced whole-cell currents of comparable amplitude, that were abolished by the N-methyl-d-aspartate (NMDA) receptor antagonist 2-amino-5-phosphonovalerate (AP-5) (20 μM). AMPA (100 μM) induced whole-cell currents which were not modified by AP-5 (20 μM) but were blocked by CNQX. In the outside-out configuration, both l-HC and d-HC (15 μM) elicited single-channel currents of the same conductance, mean open time and reversal potential as the NMDA-induced single-channel events. In the presence of Mg2+ (2 mM), d- and l-HC-induced single-channel currents were voltage-dependent. These data suggest that in cerebellar granule cells in culture, both l-HC and d-HC activate the same NMDA receptor channel complex.

Original languageEnglish (US)
Pages (from-to)231-235
Number of pages5
JournalNeuroscience letters
Volume141
Issue number2
DOIs
StatePublished - Jul 20 1992

Keywords

  • AMPA
  • AP-5
  • Cerebellum
  • Granule cell
  • NMDA
  • Patch-clamp
  • d-Homocysteic acid
  • l-Homocysteic acid

ASJC Scopus subject areas

  • Neuroscience(all)

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