Abstract
The whole-cell and the outside-out configurations of the patch-clamp technique were used to study macroscopic and single-channel currents evoked by the enantiomers of homocysteic acid, the sulphur containing analogue of glutamate, in cerebellar granule cells in culture. l-Homocysteic acid (l-HC, 15 μM) and d-homocysteic acid (d-HC, 50 μM) induced whole-cell currents of comparable amplitude, that were abolished by the N-methyl-d-aspartate (NMDA) receptor antagonist 2-amino-5-phosphonovalerate (AP-5) (20 μM). AMPA (100 μM) induced whole-cell currents which were not modified by AP-5 (20 μM) but were blocked by CNQX. In the outside-out configuration, both l-HC and d-HC (15 μM) elicited single-channel currents of the same conductance, mean open time and reversal potential as the NMDA-induced single-channel events. In the presence of Mg2+ (2 mM), d- and l-HC-induced single-channel currents were voltage-dependent. These data suggest that in cerebellar granule cells in culture, both l-HC and d-HC activate the same NMDA receptor channel complex.
Original language | English (US) |
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Pages (from-to) | 231-235 |
Number of pages | 5 |
Journal | Neuroscience letters |
Volume | 141 |
Issue number | 2 |
DOIs | |
State | Published - Jul 20 1992 |
Keywords
- AMPA
- AP-5
- Cerebellum
- Granule cell
- NMDA
- Patch-clamp
- d-Homocysteic acid
- l-Homocysteic acid
ASJC Scopus subject areas
- General Neuroscience