Site-directed mutants of rat testis fructose 6-phosphate, 2- kinase/fructose 2,6-bisphosphatase: Localization of conformational alterations induced by ligand binding

Michael K. Helms, Theodore L. Hazlett, Hiroyuki Mizuguchi, Charles A. Hasemann, Kosaku Uyeda, David M. Jameson

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Abstract

Site-directed mutagenesis was utilized to construct mutants, containing one or two tryptophan residues, of the bifunctional enzyme fructose 6- phosphate,2-kinase-fructose 2,6-bisphosphatase. Two of the single-tryptophan mutants (W15 and W64) had the tryptophan residue located in the kinase domain, which is in the N-terminal half, and two (W299 and W320) had the tryptophan residue located in the phosphatase domain, which is in the C- terminal half. The double-tryptophan mutants were W15/W64, W15/W299, W64/W299, and W299/W320. Dynamic polarization data indicated that these tryptophan residues had varying degrees of local mobility. Steady-state polarization data revealed energy transfer between the tryptophan residues in the double mutant W299/W320 but not in the W15/W64, W15/W299, or W64/W299 mutants, indicating the proximity of the W299 and W320 residues. The binding of fructose-6-phosphate resulted in a significant increase in the anisotropy of the W15 mutants, but did not affect the anisotropies of any of the other single-tryptophan mutants. Binding of fructose-2,6-bisphosphate also significantly increased the anisotropy of W15. In the case of fructose-6- phosphate binding, the increased anisotropy was shown to be due to a restriction of the tryptophan residue's local mobility in the presence of bound ligand, which suggests that the N-terminus is located near the kinase active site. These increases in anisotropies were used to estimate the dissociation constants of fructose-6-phosphate and fructose-2,6- bisphosphate, which were 29 ± 3 and 2.1 ± 0.3 μM, respectively. These observations are considered in light of the recently published crystal structure for this bifunctional enzyme.

Original languageEnglish (US)
Pages (from-to)14057-14064
Number of pages8
JournalBiochemistry
Volume37
Issue number40
DOIs
StatePublished - Oct 6 1998

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ASJC Scopus subject areas

  • Biochemistry

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