Site-specific analysis of inflammatory markers in discoid lupus erythematosus skin

Ryan B. Thorpe, Anna Gray, Kirthi R. Kumar, Joseph S. Susa, Benjamin F. Chong

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Prior studies identified T cells, B cells, and macrophages in the inflammatory infiltrate and up-regulation of their protein products in discoid lupus erythematosus (DLE) skin; however, they lacked rigorous analyses to define their specific locations in skin. Thus, we compared expressions of selected T cell, B cell, and macrophage markers in five areas of DLE, psoriasis, and normal skin. Immunostainings for CD3, CD4, CD8, CD20, CD68, CXCR3, CXCL10, and TIA-1 were performed in biopsies of 23 DLE lesional skin, 11 psoriasis lesional skin, and 5 normal skin. Three independent observers used a graded scale to rate each marker's presence in the epidermis, dermatoepidermal junction (DEJ), perivascular area, periadnexal area, and deep dermis. DLE lesional skin contained an increased abundance of CD3+, CD8+, and CD68+ cells at the DEJ, and CD20+ and CD68+ cells in the periadnexal area versus psoriasis and normal skin. CXCR3, CXCL10, and TIA-1 were elevated in periadnexal sites of DLE lesional skin versus psoriasis lesional skin. The aggregation of T cells, B cells, macrophages, and their protein products (CXCR3, CXCL10, and TIA-1) in the DEJ and periadnexal area of DLE lesional skin may contribute to the pathology of DLE through a coordinated, sophisticated process.

Original languageEnglish (US)
Article number925805
JournalThe Scientific World Journal
Volume2014
DOIs
StatePublished - 2014

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Discoid Lupus Erythematosus
skin
Skin
Psoriasis
T-cells
Macrophages
B-Lymphocytes
Cells
T-Lymphocytes
analysis
marker
Biopsy
Pathology
Dermis
pathology
Epidermis
Proteins
Up-Regulation
Agglomeration

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Site-specific analysis of inflammatory markers in discoid lupus erythematosus skin. / Thorpe, Ryan B.; Gray, Anna; Kumar, Kirthi R.; Susa, Joseph S.; Chong, Benjamin F.

In: The Scientific World Journal, Vol. 2014, 925805, 2014.

Research output: Contribution to journalArticle

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